High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues

article High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues published yes Phanu T. Serivichyaswat author Kai Bartusch author 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a0000-0003-2708-5545 Martina Leso author Constance Musseau author Akira Iwase author Yu Chen author Keiko Sugimoto author Marcel Quint author Charles W. Melnyk author Cellular regeneration in response to wounding is fundamental to maintain tissue integrity. Various internal factors including hormones and transcription factors mediate healing, but little is known about the role of external factors. To understand how the environment affects regeneration, we investigated the effects of temperature upon the horticulturally relevant process of plant grafting. We found that elevated temperatures accelerated vascular regeneration in Arabidopsis thaliana and tomato grafts. Leaves were crucial for this effect, as blocking auxin transport or mutating PHYTOCHROME INTERACTING FACTOR 4 (PIF4) or YUCCA2/5/8/9 in the cotyledons abolished the temperature enhancement. However, these perturbations did not affect grafting at ambient temperatures, and temperature enhancement of callus formation and tissue adhesion did not require PIF4, suggesting leaf-derived auxin specifically enhanced vascular regeneration in response to elevated temperatures. We also found that elevated temperatures accelerated the formation of inter-plant vascular connections between the parasitic plant Phtheirospermum japonicum and host Arabidopsis, and this effect required shoot-derived auxin from the parasite. Taken together, our results identify a pathway whereby local temperature perception mediates long distance auxin signaling to modify regeneration, grafting and parasitism. The Company of Biologists2022 eng Development 0950-1991 1477-9129 3521785710.1242/dev.200079 1495 yes Serivichyaswat, Phanu T., Kai Bartusch, Martina Leso, Constance Musseau, Akira Iwase, Yu Chen, Keiko Sugimoto, Marcel Quint, and Charles W. Melnyk. “High Temperature Perception in Leaves Promotes Vascular Regeneration and Graft Formation in Distant Tissues.” Development. The Company of Biologists, 2022. <a href="https://doi.org/10.1242/dev.200079">https://doi.org/10.1242/dev.200079</a>. P.T. Serivichyaswat, K. Bartusch, M. Leso, C. Musseau, A. Iwase, Y. Chen, K. Sugimoto, M. Quint, C.W. Melnyk, Development 149 (2022). Serivichyaswat, Phanu T., et al. “High Temperature Perception in Leaves Promotes Vascular Regeneration and Graft Formation in Distant Tissues.” Development, vol. 149, no. 5, dev200079, The Company of Biologists, 2022, doi:<a href="https://doi.org/10.1242/dev.200079">10.1242/dev.200079</a>. P. T. Serivichyaswat et al., “High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues,” Development, vol. 149, no. 5. The Company of Biologists, 2022. Serivichyaswat PT, Bartusch K, Leso M, Musseau C, Iwase A, Chen Y, Sugimoto K, Quint M, Melnyk CW. 2022. High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues. Development. 149(5), dev200079. Serivichyaswat, P. T., Bartusch, K., Leso, M., Musseau, C., Iwase, A., Chen, Y., … Melnyk, C. W. (2022). High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues. Development. The Company of Biologists. <a href="https://doi.org/10.1242/dev.200079">https://doi.org/10.1242/dev.200079</a> Serivichyaswat PT, Bartusch K, Leso M, et al. High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues. Development. 2022;149(5). doi:<a href="https://doi.org/10.1242/dev.200079">10.1242/dev.200079</a> 231322026-10-13T11:15:22Z2026-10-14T12:22:27Z