---
res:
  bibo_abstract:
  - "Background\r\nCotyledon micrografting represents a useful tool for studying the
    central role of cotyledons during early plant development, especially their interplay
    with other plant organs with regard to long distance transport. While hypocotyl
    micrografting methods are well-established, cotyledon micrografting is still inefficient.
    By optimizing cotyledon micrografting, we aim for higher success rates and increased
    throughput in the model species Arabidopsis thaliana.\r\n\r\nResults\r\nWe established
    a cut and paste cotyledon surgery procedure on a flat and solid but moist surface
    which improved handling of small seedlings. By applying a specific cutting and
    joining pattern, throughput was increased up to 40 seedlings per hour. The combination
    of short-day photoperiods and low light intensities for germination and long days
    plus high light intensities, elevated temperature and vertical plate positioning
    after grafting significantly increased ‘ligation’ efficiency. In particular high
    temperatures affected success rates favorably. Altogether, we achieved up to 92%
    grafting success in A. thaliana. Reconnection of vasculature was demonstrated
    by transport of a vasculature-specific dye across the grafting site. Phloem and
    xylem reconnection were completed 3–4 and 4–6 days after grafting, respectively,
    in a temperature-dependent manner. We observed that plants with grafted cotyledons
    match plants with intact cotyledons in biomass production and rosette development.\r\n\r\nConclusions\r\nThis
    cut and paste cotyledon-to-petiole micrografting protocol simplifies the handling
    of plant seedlings in surgery, increases the number of grafted plants per hour
    and greatly improves success rates for A. thaliana seedlings. The developed cotyledon
    micrografting method is also suitable for other plant species of comparable size.@eng"
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Kai
      foaf_name: Bartusch, Kai
      foaf_surname: Bartusch
      foaf_workInfoHomepage: http://www.librecat.org/personId=0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
    orcid: 0000-0003-2708-5545
  - foaf_Person:
      foaf_givenName: Jana
      foaf_name: Trenner, Jana
      foaf_surname: Trenner
  - foaf_Person:
      foaf_givenName: Charles W.
      foaf_name: Melnyk, Charles W.
      foaf_surname: Melnyk
  - foaf_Person:
      foaf_givenName: Marcel
      foaf_name: Quint, Marcel
      foaf_surname: Quint
  bibo_doi: 10.1186/s13007-020-0562-1
  bibo_volume: 16
  dct_date: 2020^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1746-4811
  dct_language: eng
  dct_publisher: Springer@
  dct_title: 'Cut and paste: Temperature-enhanced cotyledon micrografting for Arabidopsis
    thaliana seedlings@'
  fabio_hasPubmedId: '32042304'
...