---
res:
  bibo_abstract:
  - Small RNAs (sRNAs) are widespread across diverse flowering plant species and play
    important roles regulating reproduction and environmental responses. However,
    how sRNAs exert their function is largely unknown, likely due to their typical
    origin in non-genic regions, the lack of distinct gene targets, and the sparse
    characterization of their associated Argonaute (AGO) effector proteins. Here we
    show that Arabidopsis male reproductive sRNAs associate with AGO4, 5, 6 and 9,
    all of which are enriched in meiocytes along with RNA Polymerase V (Pol V). This
    enables these sRNAs to methylate loci with imperfect sequence homology. AGO4,
    AGO6 and AGO9 predominantly load 24-nucleotide (nt) Pol IV-derived sRNAs and mediate
    DNA methylation in meiocytes in a dosage-dependent manner. Despite closer homology
    to microRNA-associated AGO1 and AGO10, AGO5 primarily binds CLSY3- and Pol IV-derived
    21-24 nt sRNAs. Transcriptomic and methylomic analyses of meiocytes from single
    and compound ago mutants demonstrate distinct yet complementary roles of AGO4/6/9
    and AGO5 in regulating protein-coding genes and silencing transposons in the germline.
    These molecular disruptions are accompanied by significant meiotic defects. Together,
    our findings reveal how AGO4/6/9-mediated DNA methylation and AGO5, likely acting
    through post-transcriptional gene silencing, work together to promote male germline
    development.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jincheng
      foaf_name: Long, Jincheng
      foaf_surname: Long
  - foaf_Person:
      foaf_givenName: Chao
      foaf_name: Yang, Chao
      foaf_surname: Yang
      foaf_workInfoHomepage: http://www.librecat.org/personId=082e3e6e-8069-11ed-8390-c8cce7b1aaca
  - foaf_Person:
      foaf_givenName: Shujuan
      foaf_name: Xu, Shujuan
      foaf_surname: Xu
      foaf_workInfoHomepage: http://www.librecat.org/personId=9724dd9d-f591-11ee-bd51-e97ed0652286
  - foaf_Person:
      foaf_givenName: Joke G
      foaf_name: De Jaeger-Braet, Joke G
      foaf_surname: De Jaeger-Braet
      foaf_workInfoHomepage: http://www.librecat.org/personId=26bd38d3-c59a-11ee-a1af-d7a988cafcc5
  - foaf_Person:
      foaf_givenName: Wenjing
      foaf_name: She, Wenjing
      foaf_surname: She
  - foaf_Person:
      foaf_givenName: Seijiro
      foaf_name: Ono, Seijiro
      foaf_surname: Ono
  - foaf_Person:
      foaf_givenName: James
      foaf_name: Walker, James
      foaf_surname: Walker
  - foaf_Person:
      foaf_givenName: Ygor
      foaf_name: de Souza Vieira, Ygor
      foaf_surname: de Souza Vieira
      foaf_workInfoHomepage: http://www.librecat.org/personId=af019b77-d2a9-11f1-9e65-c47a5932b764
  - foaf_Person:
      foaf_givenName: Arp
      foaf_name: Schnittger, Arp
      foaf_surname: Schnittger
  - foaf_Person:
      foaf_givenName: Xiaoqi
      foaf_name: Feng, Xiaoqi
      foaf_surname: Feng
      foaf_workInfoHomepage: http://www.librecat.org/personId=e0164712-22ee-11ed-b12a-d80fcdf35958
    orcid: 0000-0002-4008-1234
  bibo_doi: 10.1038/s41467-026-77326-1
  bibo_volume: 17
  dct_date: 2026^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/2041-1723
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Multiple Argonaute complexes orchestrate epigenetic silencing in the
    Arabidopsis germline@
  fabio_hasPubmedId: '42869904'
...