--- res: bibo_abstract: - The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Valery foaf_name: Grinevich, Valery V foaf_surname: Grinevich - foaf_Person: foaf_givenName: Alexander foaf_name: Kolleker, Alexander foaf_surname: Kolleker - foaf_Person: foaf_givenName: Marina foaf_name: Eliava, Marina I foaf_surname: Eliava - foaf_Person: foaf_givenName: Naoki foaf_name: Takada, Naoki foaf_surname: Takada - foaf_Person: foaf_givenName: Hiroshi foaf_name: Takuma, Hiroshi foaf_surname: Takuma - foaf_Person: foaf_givenName: Yugo foaf_name: Fukazawa, Yugo foaf_surname: Fukazawa - foaf_Person: foaf_givenName: Ryuichi foaf_name: Ryuichi Shigemoto foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 - foaf_Person: foaf_givenName: Dietmar foaf_name: Kuhl, Dietmar foaf_surname: Kuhl - foaf_Person: foaf_givenName: Jack foaf_name: Waters, Jack foaf_surname: Waters - foaf_Person: foaf_givenName: Peter foaf_name: Seeburg, Peter H foaf_surname: Seeburg - foaf_Person: foaf_givenName: Pavel foaf_name: Osten, Pavel foaf_surname: Osten bibo_doi: 10.1016/j.jneumeth.2009.07.015 bibo_issue: '1' bibo_volume: 184 dct_date: 2009^xs_gYear dct_publisher: Elsevier@ dct_title: 'Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo@' ...