{"publisher":"Elsevier","abstract":[{"lang":"eng","text":"The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo."}],"publist_id":"4400","extern":1,"issue":"1","intvolume":"       184","doi":"10.1016/j.jneumeth.2009.07.015","citation":{"short":"V. Grinevich, A. Kolleker, M. Eliava, N. Takada, H. Takuma, Y. Fukazawa, R. Shigemoto, D. Kuhl, J. Waters, P. Seeburg, P. Osten, Journal of Neuroscience Methods 184 (2009) 25–36.","apa":"Grinevich, V., Kolleker, A., Eliava, M., Takada, N., Takuma, H., Fukazawa, Y., … Osten, P. (2009). Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. <i>Journal of Neuroscience Methods</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.jneumeth.2009.07.015\">https://doi.org/10.1016/j.jneumeth.2009.07.015</a>","mla":"Grinevich, Valery, et al. “Fluorescent Arc/Arg3.1 Indicator Mice: A Versatile Tool to Study Brain Activity Changes in Vitro and in Vivo.” <i>Journal of Neuroscience Methods</i>, vol. 184, no. 1, Elsevier, 2009, pp. 25–36, doi:<a href=\"https://doi.org/10.1016/j.jneumeth.2009.07.015\">10.1016/j.jneumeth.2009.07.015</a>.","ista":"Grinevich V, Kolleker A, Eliava M, Takada N, Takuma H, Fukazawa Y, Shigemoto R, Kuhl D, Waters J, Seeburg P, Osten P. 2009. Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods. 184(1), 25–36.","ama":"Grinevich V, Kolleker A, Eliava M, et al. Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. <i>Journal of Neuroscience Methods</i>. 2009;184(1):25-36. doi:<a href=\"https://doi.org/10.1016/j.jneumeth.2009.07.015\">10.1016/j.jneumeth.2009.07.015</a>","ieee":"V. Grinevich <i>et al.</i>, “Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo,” <i>Journal of Neuroscience Methods</i>, vol. 184, no. 1. Elsevier, pp. 25–36, 2009.","chicago":"Grinevich, Valery, Alexander Kolleker, Marina Eliava, Naoki Takada, Hiroshi Takuma, Yugo Fukazawa, Ryuichi Shigemoto, et al. “Fluorescent Arc/Arg3.1 Indicator Mice: A Versatile Tool to Study Brain Activity Changes in Vitro and in Vivo.” <i>Journal of Neuroscience Methods</i>. Elsevier, 2009. <a href=\"https://doi.org/10.1016/j.jneumeth.2009.07.015\">https://doi.org/10.1016/j.jneumeth.2009.07.015</a>."},"publication_status":"published","year":"2009","type":"journal_article","day":"30","status":"public","author":[{"first_name":"Valery","last_name":"Grinevich","full_name":"Grinevich, Valery V"},{"first_name":"Alexander","last_name":"Kolleker","full_name":"Kolleker, Alexander"},{"full_name":"Eliava, Marina I","last_name":"Eliava","first_name":"Marina"},{"full_name":"Takada, Naoki","last_name":"Takada","first_name":"Naoki"},{"full_name":"Takuma, Hiroshi","last_name":"Takuma","first_name":"Hiroshi"},{"last_name":"Fukazawa","full_name":"Fukazawa, Yugo","first_name":"Yugo"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Ryuichi Shigemoto","last_name":"Shigemoto","orcid":"0000-0001-8761-9444"},{"full_name":"Kuhl, Dietmar","last_name":"Kuhl","first_name":"Dietmar"},{"full_name":"Waters, Jack","last_name":"Waters","first_name":"Jack"},{"full_name":"Seeburg, Peter H","last_name":"Seeburg","first_name":"Peter"},{"first_name":"Pavel","full_name":"Osten, Pavel","last_name":"Osten"}],"date_published":"2009-10-30T00:00:00Z","_id":"2501","quality_controlled":0,"title":"Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo","date_created":"2018-12-11T11:58:02Z","publication":"Journal of Neuroscience Methods","page":"25 - 36","date_updated":"2021-01-12T06:57:52Z","month":"10","volume":184}