{"publisher":"American Society for Biochemistry and Molecular Biology","abstract":[{"lang":"eng","text":"A cDNA clone for a new metabotropic glutamate receptor, termed mGluR6, was isolated from a rat retinal cDNA library by cross-hybridization with the previously isolated cDNA clone for a metabotropic glutamate receptor. The cloned mGluR6 subtype consists of 871 amino acid residues and exhibits a structural architecture common to the metabotropic receptor family, possessing a large extracellular domain preceding the seven putative membrane-spanning domains. mGluR6 shows the highest sequence similarity to mGluR4 among the metabotropic receptor subtypes and inhibits the forskolin- stimulated cyclic AMP accumulation in Chinese hamster ovary cells transfected with the cloned cDNA. mGluR6 potently reacts with L-2-amino-4- phosphonobutyrate (L-AP4) and L-serine-O-phosphate, and the potencies of these compounds are one order of magnitude greater than that of L-glutamate. Blot and in situ hybridization analyses indicated that mGluR6 mRNA is restrictedly expressed in the inner nuclear layer of the retina where ON- bipolar cells are distributed. The metabotropic receptor that responds strongly to L-AP4 and L-serine-O-phosphate in ON-bipolar cells is known to mediate glutamate synaptic transmission between photoreceptor cells and ON- bipolar cells. On the basis of the agonist selectivity of mGluR6 and its specific expression in retinal cells, the physiological role of this receptor subtype in the visual system is discussed."}],"publist_id":"4362","article_processing_charge":"No","language":[{"iso":"eng"}],"issue":"16","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/S0021-9258(19)50280-0","scopus_import":"1","citation":{"chicago":"Nakajima, Yoshiaki, Hideki Iwakabe, Chihiro Akazawa, Hiroyuki Nawa, Ryuichi Shigemoto, Noboru Mizuno, and Shigetada Nakanishi. “Molecular Characterization of a Novel Retinal Metabotropic Glutamate Receptor MGluR6 with a High Agonist Selectivity for L-2-Amino-4- Phosphonobutyrate.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 1993. https://doi.org/10.1016/S0021-9258(19)50280-0.","ama":"Nakajima Y, Iwakabe H, Akazawa C, et al. Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4- phosphonobutyrate. Journal of Biological Chemistry. 1993;268(16):11868-11873. doi:10.1016/S0021-9258(19)50280-0","ieee":"Y. Nakajima et al., “Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4- phosphonobutyrate,” Journal of Biological Chemistry, vol. 268, no. 16. American Society for Biochemistry and Molecular Biology, pp. 11868–11873, 1993.","short":"Y. Nakajima, H. Iwakabe, C. Akazawa, H. Nawa, R. Shigemoto, N. Mizuno, S. Nakanishi, Journal of Biological Chemistry 268 (1993) 11868–11873.","apa":"Nakajima, Y., Iwakabe, H., Akazawa, C., Nawa, H., Shigemoto, R., Mizuno, N., & Nakanishi, S. (1993). Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4- phosphonobutyrate. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1016/S0021-9258(19)50280-0","ista":"Nakajima Y, Iwakabe H, Akazawa C, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. 1993. Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4- phosphonobutyrate. Journal of Biological Chemistry. 268(16), 11868–11873.","mla":"Nakajima, Yoshiaki, et al. “Molecular Characterization of a Novel Retinal Metabotropic Glutamate Receptor MGluR6 with a High Agonist Selectivity for L-2-Amino-4- Phosphonobutyrate.” Journal of Biological Chemistry, vol. 268, no. 16, American Society for Biochemistry and Molecular Biology, 1993, pp. 11868–73, doi:10.1016/S0021-9258(19)50280-0."},"day":"05","status":"public","date_published":"1993-06-05T00:00:00Z","publication_identifier":{"issn":["0021-9258"]},"main_file_link":[{"url":"https://doi.org/10.1016/S0021-9258(19)50280-0","open_access":"1"}],"quality_controlled":"1","external_id":{"pmid":["8389366"]},"publication":"Journal of Biological Chemistry","extern":"1","intvolume":" 268","article_type":"original","acknowledgement":"This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Ministry of Health and Welfare, the Yamanouchi Foundation for Research on Metabolic Disorders, the Uehara Memorial Foundation, and the Inamori Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. \r\n\r\nWe are grateful to Akira Uesugi for photographic assistance.","oa_version":"Published Version","oa":1,"pmid":1,"publication_status":"published","year":"1993","type":"journal_article","author":[{"first_name":"Yoshiaki","last_name":"Nakajima","full_name":"Nakajima, Yoshiaki"},{"first_name":"Hideki","full_name":"Iwakabe, Hideki","last_name":"Iwakabe"},{"first_name":"Chihiro","full_name":"Akazawa, Chihiro","last_name":"Akazawa"},{"full_name":"Nawa, Hiroyuki","last_name":"Nawa","first_name":"Hiroyuki"},{"full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Noboru","full_name":"Mizuno, Noboru","last_name":"Mizuno"},{"full_name":"Nakanishi, Shigetada","last_name":"Nakanishi","first_name":"Shigetada"}],"_id":"2536","title":"Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4- phosphonobutyrate","date_created":"2018-12-11T11:58:15Z","date_updated":"2022-04-26T06:56:15Z","month":"06","page":"11868 - 11873","volume":268}