---
_id: '2564'
abstract:
- lang: eng
text: The distribution of the neuromedin K receptor (NK3; NKR) in the central nervous
system was investigated in the adult rat by using in situ hybridization and immunohistochemical
techniques. The rabbit anti-NKR antibody was raised against a bacterial fusion
protein containing a C- terminal portion of NKR and affinity purified with a Sepharose
4B column conjugated to the fusion protein. Immunoblot analysis was performed
to test the reactivity and specificity of the antibody. Crude membrane was prepared
from cDNA-transfected Chinese hamster ovary (CHO) cells expressing each of the
rat NKR, substance P receptor (NK1; SPR), and substance K receptor (NK2; SKR)
and from the hypothalamus, cerebral cortex, and cerebellum. Immunoreactive bands
were observed specifically in the NKR-CHO cells, hypothalamus, and cerebral cortex
but not in the SPR- or SKR-CHO cells, nor in the cerebellum. Molecular weights
of the immunoreactive bands ranged from 73 to 89 kDa and from 59 to 83 kDa in
the NKR-CHO cells and tissues, respectively. The distribution of NKR-like immunoreactivity
coincided with that of NKR mRNA. The expression of NKR was indicated on neuronal
cell bodies and dendrites. NKR was found to be expressed intensely or moderately
in neurons in the glomerular and granule cell layers of the main olfactory bulb;
glomerular and mitral cell layers of the accessory olfactory bulb; layers IV and
V of the cerebral neocortex; medial septal nucleus; nucleus of the diagonal band;
bed nucleus of the stria terminalis; globus pallidus; ventral pallidum; paraventricular
nucleus; supraoptic nucleus; zona incerta; dorsal, lateral, and posterior hypothalamic
areas; amygdaloid nuclei; medial habenular nucleus; ventral tegmental area; midbrain
periaqueductal gray; interpeduncular nuclei; substantia nigra pars compacta; linear,
median, dorsal, and pontine raphe nuclei; posteromedial tegmental nucleus; sphenoid
nucleus; nucleus of the solitary tract; intermediate and rostroventrolateral reticular
nuclei; and lamina II of the caudal spinal trigeminal nucleus and spinal dorsal
horn. These findings are discussed in relation to the physiological functions
associated with neuromedin K.
acknowledgement: 'We are grateful for the photographic help of Mr. A. Uesugi and
the support of Drs. Satoru Fukuchi, Ritsu Hayashi, Sozaburo Hayashi, Mizuho Katsurada,
Yutaka Kitani, Keiko Kumagai, Toshihiko Kuroda, Hiroshi Matsu- bara, Hiroshi Matsushima, Chisato
Minakuchi, Gonpei Niwa, Hajime Oda, Masahiko Ohbayashi, Sei-ichi Ohbayashi,
Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watanabe, Kazuo Yoshino, and Toshiaki Yoshino.
This work was supported in part by Grants-in-Aid for Special Research on Priority
areas 05267104, Scientific Research (B) 05454658, and Scientific Research (C) 06680735
from the Ministry of Education, Science and Culture of Japan. '
article_processing_charge: No
article_type: original
author:
- first_name: Yu
full_name: Ding, Yu
last_name: Ding
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Masahiko
full_name: Takada, Masahiko
last_name: Takada
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: Ding Y, Shigemoto R, Takada M, Ohishi H, Nakanishi S, Mizuno N. Localization
of the neuromedin K receptor (NK3) in the central nervous system of the rat. Journal
of Comparative Neurology. 1996;364(2):290-310. doi:10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
apa: Ding, Y., Shigemoto, R., Takada, M., Ohishi, H., Nakanishi, S., & Mizuno,
N. (1996). Localization of the neuromedin K receptor (NK3) in the central nervous
system of the rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
chicago: Ding, Yu, Ryuichi Shigemoto, Masahiko Takada, Hitoshi Ohishi, Shigetada
Nakanishi, and Noboru Mizuno. “Localization of the Neuromedin K Receptor (NK3)
in the Central Nervous System of the Rat.” Journal of Comparative Neurology.
Wiley-Blackwell, 1996. https://doi.org/10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0.
ieee: Y. Ding, R. Shigemoto, M. Takada, H. Ohishi, S. Nakanishi, and N. Mizuno,
“Localization of the neuromedin K receptor (NK3) in the central nervous system
of the rat,” Journal of Comparative Neurology, vol. 364, no. 2. Wiley-Blackwell,
pp. 290–310, 1996.
ista: Ding Y, Shigemoto R, Takada M, Ohishi H, Nakanishi S, Mizuno N. 1996. Localization
of the neuromedin K receptor (NK3) in the central nervous system of the rat. Journal
of Comparative Neurology. 364(2), 290–310.
mla: Ding, Yu, et al. “Localization of the Neuromedin K Receptor (NK3) in the Central
Nervous System of the Rat.” Journal of Comparative Neurology, vol. 364,
no. 2, Wiley-Blackwell, 1996, pp. 290–310, doi:10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0.
short: Y. Ding, R. Shigemoto, M. Takada, H. Ohishi, S. Nakanishi, N. Mizuno, Journal
of Comparative Neurology 364 (1996) 290–310.
date_created: 2018-12-11T11:58:25Z
date_published: 1996-01-08T00:00:00Z
date_updated: 2022-08-12T09:48:24Z
day: '08'
doi: 10.1002/(SICI)1096-9861(19960108)364:2<290::AID-CNE8>3.0.CO;2-0
extern: '1'
external_id:
pmid:
- '8788251 '
intvolume: ' 364'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
page: 290 - 310
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4334'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Localization of the neuromedin K receptor (NK3) in the central nervous system
of the rat
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 364
year: '1996'
...