{"abstract":[{"text":"The distribution of immunoreactivity to the neurokinin3 receptor (NK3R) was examined in segments C7, T11-12, L1-2, and L4-6 of the rat spinal cord. NK3R immunoreactivity was visualized by using two antisera generated against sequences of amino acids contained in the C-terminal region of the NK3R. NK3R-immunoreactive cells were numerous in the substantia gelatinosa of all spinal segments examined as well as the dorsal commissural nucleus of spinal segments L1-2. Isolated, immunoreactive cells were scattered throughout other regions of the spinal cord. The relationship of NK3R-immunoreactivity with neurons was demonstrated by colocalization with microtubule associated protein 2-immunoreactivity in individual cells. Within neurons, NK3R- immunoreactivity was associated predominately with the plasma membrane of cell bodies and dendrites. Within the substantia gelatinosa, 86% of nitric oxide synthase (NOS)-immunoreactive neurons were also NK3R-immunoreactive. Although NOS-immunoreactive neurons were found throughout all other regions of the spinal cord in the segments examined, these were not NK3R- immunoreactive. When preganglionic sympathetic neurons in spinal segments T11-12 and L1-2 were visualized by intraperitoneal injection of Fluorogold, less than 1% of the Fluorogold-labeled neurons were also immunoreactive for NK3R. The large number of NK3R-immunoreactive neurons in the substantia gelatinosa suggests that some effects of tachykinins an somatosensation may be mediated by NK3R.","lang":"eng"}],"doi":"10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3","year":"1997","date_created":"2018-12-11T11:58:29Z","citation":{"short":"V. Seybold, I. Grković, A. Portbury, Y. Ding, R. Shigemoto, N. Mizuno, J. Furness, B. Southwell, Journal of Comparative Neurology 381 (1997) 439–448.","ieee":"V. Seybold et al., “Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons in rat spinal cord,” Journal of Comparative Neurology, vol. 381, no. 4. Wiley-Blackwell, pp. 439–448, 1997.","ama":"Seybold V, Grković I, Portbury A, et al. Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons in rat spinal cord. Journal of Comparative Neurology. 1997;381(4):439-448. doi:10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3","chicago":"Seybold, Virginia, Ivica Grković, Andrea Portbury, Yu Ding, Ryuichi Shigemoto, Noboru Mizuno, John Furness, and Bridget Southwell. “Relationship of NK3 Receptor-Immunoreactivity to Subpopulations of Neurons in Rat Spinal Cord.” Journal of Comparative Neurology. Wiley-Blackwell, 1997. https://doi.org/10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3.","apa":"Seybold, V., Grković, I., Portbury, A., Ding, Y., Shigemoto, R., Mizuno, N., … Southwell, B. (1997). Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons in rat spinal cord. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3","mla":"Seybold, Virginia, et al. “Relationship of NK3 Receptor-Immunoreactivity to Subpopulations of Neurons in Rat Spinal Cord.” Journal of Comparative Neurology, vol. 381, no. 4, Wiley-Blackwell, 1997, pp. 439–48, doi:10.1002/(SICI)1096-9861(19970519)381:4<439::AID-CNE4>3.0.CO;2-3.","ista":"Seybold V, Grković I, Portbury A, Ding Y, Shigemoto R, Mizuno N, Furness J, Southwell B. 1997. Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons in rat spinal cord. Journal of Comparative Neurology. 381(4), 439–448."},"publication":"Journal of Comparative Neurology","article_processing_charge":"No","publist_id":"4320","day":"19","type":"journal_article","oa_version":"None","article_type":"original","publication_status":"published","pmid":1,"scopus_import":"1","issue":"4","publication_identifier":{"issn":["0021-9967"]},"publisher":"Wiley-Blackwell","page":"439 - 448","volume":381,"extern":"1","status":"public","acknowledgement":"The authors are grateful to Dr. Colin Anderson fordiscussions of the organization of spinal autonomic nuclei.V.S.S. was a visiting research fellow in the Department ofAnatomy and Cell Biology, University of Melbourne.","language":[{"iso":"eng"}],"quality_controlled":"1","date_updated":"2022-08-22T12:27:14Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","external_id":{"pmid":["9136801 "]},"intvolume":" 381","month":"05","date_published":"1997-05-19T00:00:00Z","title":"Relationship of NK3 receptor-immunoreactivity to subpopulations of neurons in rat spinal cord","author":[{"last_name":"Seybold","full_name":"Seybold, Virginia","first_name":"Virginia"},{"first_name":"Ivica","full_name":"Grković, Ivica","last_name":"Grković"},{"first_name":"Andrea","last_name":"Portbury","full_name":"Portbury, Andrea"},{"full_name":"Ding, Yu","last_name":"Ding","first_name":"Yu"},{"orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"},{"first_name":"John","full_name":"Furness, John","last_name":"Furness"},{"first_name":"Bridget","full_name":"Southwell, Bridget","last_name":"Southwell"}],"_id":"2578"}