---
_id: '2592'
abstract:
- lang: eng
text: Metabotropic glutamate receptors (mGluRs) consist of eight different subtypes
and exert their effects or second messengers and ion channels via G- proteins.
The function of individual mGluR subtypes in the CNS, however, largely remains
to be clarified. We examined the fear response of freezing after electric shock
in wild-type and mGluR7(-/-) knockout littermates. Wild- type mice displayed freezing
immediately after and 1 d after footshock. In comparison, mGluR7(-/-) knockout
mice showed significantly reduced levels in both immediate postshock and delayed
freezing responses. However, the knockout mice exhibited no abnormalities in pain
sensitivity and locomotor activity. To further examine amygdala-dependent behavior,
we performed conditioned taste aversion (CTA) experiments. In wild-type mice,
the administration of saccharin followed by intraperitoneal injection of the malaise-inducing
agent LiCl resulted in an association between saccharin and LiCl. This association
caused strong CTA toward saccharin n contrast, mGluR7(-/-) knockout mice failed
to associate between the taste and the negative reinforcer in CTA experiments.
Again, the knockout mice showed no abnormalities in taste preference and in the
sensitivity to LiCl toxicity. These results indicate that mGluR7 deficiency causes
an impairment of two distinct amygdala-dependent behavioral paradigms. Immunohistochemical
and immunoelectron-microscopic analyses showed that mGluR7 is highly expressed
in amygdala and preferentially localized at the presynaptic axon terminals of
glutamatergic neurons. Together, these findings strongly suggest that mGluR7 is
involved in neural processes subserving amygdala-dependent averse responses.
acknowledgement: This work was supported in part by research grants from the Ministry
of Education, Science and Culture of Japan, the Ministry of Health and Welfare of
Japan, the Sankyo Foundation, the Yamanouchi Foundation, and the Biomolecular Engineering
Research Institute. We thank Takashi Yamamoto for advice on CTA experiments, Fumitaka
Ushikubi for advice on the nociception test, Markus Schroeder for back-crossing
of mutant mice, Ayae Kinoshita for the kind gift of antibodies, Akira Uesugi for
photography, and Kumlesh K. Dev for careful reading of this manuscript.
article_processing_charge: No
article_type: original
author:
- first_name: Miwako
full_name: Masugi, Miwako
last_name: Masugi
- first_name: Mineto
full_name: Yokoi, Mineto
last_name: Yokoi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Keiko
full_name: Muguruma, Keiko
last_name: Muguruma
- first_name: Yasuyoshi
full_name: Watanabe, Yasuyoshi
last_name: Watanabe
- first_name: Gilles
full_name: Sansig, Gilles
last_name: Sansig
- first_name: Herman
full_name: Van Der Putten, Herman
last_name: Van Der Putten
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
citation:
ama: Masugi M, Yokoi M, Shigemoto R, et al. Metabotropic glutamate receptor subtype
7 ablation causes deficit in fear response and conditioned taste aversion. Journal
of Neuroscience. 1999;19(3):955-963. doi:10.1523/JNEUROSCI.19-03-00955.1999
apa: Masugi, M., Yokoi, M., Shigemoto, R., Muguruma, K., Watanabe, Y., Sansig, G.,
… Nakanishi, S. (1999). Metabotropic glutamate receptor subtype 7 ablation causes
deficit in fear response and conditioned taste aversion. Journal of Neuroscience.
Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.19-03-00955.1999
chicago: Masugi, Miwako, Mineto Yokoi, Ryuichi Shigemoto, Keiko Muguruma, Yasuyoshi
Watanabe, Gilles Sansig, Herman Van Der Putten, and Shigetada Nakanishi. “Metabotropic
Glutamate Receptor Subtype 7 Ablation Causes Deficit in Fear Response and Conditioned
Taste Aversion.” Journal of Neuroscience. Society for Neuroscience, 1999.
https://doi.org/10.1523/JNEUROSCI.19-03-00955.1999.
ieee: M. Masugi et al., “Metabotropic glutamate receptor subtype 7 ablation
causes deficit in fear response and conditioned taste aversion,” Journal of
Neuroscience, vol. 19, no. 3. Society for Neuroscience, pp. 955–963, 1999.
ista: Masugi M, Yokoi M, Shigemoto R, Muguruma K, Watanabe Y, Sansig G, Van Der
Putten H, Nakanishi S. 1999. Metabotropic glutamate receptor subtype 7 ablation
causes deficit in fear response and conditioned taste aversion. Journal of Neuroscience.
19(3), 955–963.
mla: Masugi, Miwako, et al. “Metabotropic Glutamate Receptor Subtype 7 Ablation
Causes Deficit in Fear Response and Conditioned Taste Aversion.” Journal of
Neuroscience, vol. 19, no. 3, Society for Neuroscience, 1999, pp. 955–63,
doi:10.1523/JNEUROSCI.19-03-00955.1999.
short: M. Masugi, M. Yokoi, R. Shigemoto, K. Muguruma, Y. Watanabe, G. Sansig, H.
Van Der Putten, S. Nakanishi, Journal of Neuroscience 19 (1999) 955–963.
date_created: 2018-12-11T11:58:33Z
date_published: 1999-02-01T00:00:00Z
date_updated: 2023-03-27T10:00:42Z
day: '01'
doi: 10.1523/JNEUROSCI.19-03-00955.1999
extern: '1'
external_id:
pmid:
- '9920659'
fulldoi: https://doi.org/10.1523/JNEUROSCI.19-03-00955.1999
intvolume: ' 19'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782134/
month: '02'
oa: 1
oa_version: Published Version
page: 955 - 963
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '4306'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Metabotropic glutamate receptor subtype 7 ablation causes deficit in fear response
and conditioned taste aversion
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 19
year: '1999'
...