article published yes Xiao Yu author En Zhang author Arthur Craig author Ryuichi Shigemoto author 499F3ABC-F248-11E8-B48F-1D18A9856A870000-0001-8761-9444 Alfredo Ribeiro Da Silva author Yves De Koninck author In cat and monkey, lamina I cells can be classified into three basic morphological types (fusiform, pyramidal, and multipolar), and recent intracellular labeling evidence in the cat indicates that fusiform and multipolar lamina I cells are two different types of nociceptive cells, whereas pyramidal cells are innocuous thermoreceptive-specific. Because earlier observations indicated that only nociceptive dorsal horn neurons respond to substance P (SP), we examined which morphological types of lamina I neurons express receptors for SP (NK-1r). We categorized NK-1r- immunoreactive (IR) lamina I neurons in serial horizontal sections from the cervical and lumbar enlargements of four monkeys. Consistent results were obtained by two independent teams of observers. Nearly all NK-1r-IR cells were fusiform (42%) or multipolar (43%), but only 6% were pyramidal (with 9% unclassified). We obtained similar findings in three monkeys in which we used double-labeling immunocytochemistry to identify NK-1r-IR and spinothalamic lamina I neurons retrogradely labeled with cholera toxin subunit b from the thalamus; most NK-1r-IR lamina I spinothalamic neurons were fusiform (48%) or multipolar (33%), and only 10% were pyramidal. In contrast, most (~75%) pyramidal and some (~25%) fusiform and multipolar lamina I spinothalamic neurons did not display NK-1r immunoreactivity. These data indicate that most fusiform and multipolar lamina I neurons in the monkey can express NK-1r, consistent with the idea that both types are nociceptive, whereas only a small proportion of lamina I pyramidal cells express this receptor, consistent with the previous finding that they are nonnociceptive. However, these findings also indicate that not all nociceptive lamina I neurons express receptors for SP. Society for Neuroscience1999 eng 0270-6474 1021231410.1523/JNEUROSCI.19-09-03545.1999 1993545 - 3555 yes Yu, Xiao, En Zhang, Arthur Craig, Ryuichi Shigemoto, Alfredo Ribeiro Da Silva, and Yves De Koninck. “NK-1 Receptor Immunoreactivity in Distinct Morphological Types of Lamina I Neurons of the Primate Spinal Cord.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 1999. <a href="https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999">https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999</a>. Yu, Xiao, et al. “NK-1 Receptor Immunoreactivity in Distinct Morphological Types of Lamina I Neurons of the Primate Spinal Cord.” <i>Journal of Neuroscience</i>, vol. 19, no. 9, Society for Neuroscience, 1999, pp. 3545–55, doi:<a href="https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999">10.1523/JNEUROSCI.19-09-03545.1999</a>. Yu X, Zhang E, Craig A, Shigemoto R, Ribeiro Da Silva A, De Koninck Y. NK-1 receptor immunoreactivity in distinct morphological types of lamina I neurons of the primate spinal cord. <i>Journal of Neuroscience</i>. 1999;19(9):3545-3555. doi:<a href="https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999">10.1523/JNEUROSCI.19-09-03545.1999</a> Yu X, Zhang E, Craig A, Shigemoto R, Ribeiro Da Silva A, De Koninck Y. 1999. NK-1 receptor immunoreactivity in distinct morphological types of lamina I neurons of the primate spinal cord. Journal of Neuroscience. 19(9), 3545–3555. X. Yu, E. Zhang, A. Craig, R. Shigemoto, A. Ribeiro Da Silva, Y. De Koninck, Journal of Neuroscience 19 (1999) 3545–3555. X. Yu, E. Zhang, A. Craig, R. Shigemoto, A. Ribeiro Da Silva, and Y. De Koninck, “NK-1 receptor immunoreactivity in distinct morphological types of lamina I neurons of the primate spinal cord,” <i>Journal of Neuroscience</i>, vol. 19, no. 9. Society for Neuroscience, pp. 3545–3555, 1999. Yu, X., Zhang, E., Craig, A., Shigemoto, R., Ribeiro Da Silva, A., &#38; De Koninck, Y. (1999). NK-1 receptor immunoreactivity in distinct morphological types of lamina I neurons of the primate spinal cord. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href="https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999">https://doi.org/10.1523/JNEUROSCI.19-09-03545.1999</a> 25932018-12-11T11:58:34Z2023-03-27T09:54:40Z