article published yes Gilles Sansig author Trevor Bushell author Vernon Clarke author Andrei Rozov author Nail Burnashev author Chantal Portet author Fabrizio Gasparini author Markus Schmutz author Klaus Klebs author Ryuichi Shigemoto author 499F3ABC-F248-11E8-B48F-1D18A9856A870000-0001-8761-9444 Peter Flor author Rainer Kühn author Thomas Knoepfel author Markus Schroeder author David Hampson author Valerie Collett author Congxiao Zhang author Robert Duvoisin author Graham Collingridge author Herman Van Der Putten author To study the role of mGlu7 receptors (mGluR7), we used homologous recombination to generate mice lacking this metabotropic receptor subtype (mGluR7 -/-). After the serendipitous discovery of a sensory stimulus-evoked epileptic phenotype, we tested two convulsant drugs, pentylenetetrazole (PTZ) and bicuculline. In animals aged 12 weeks and older, subthreshold doses of these drugs induced seizures in mGluR7 -/-, but not in mGluR7 +/-, mice. PTZ-induced seizures were inhibited by three standard anticonvulsant drugs, but not by the group III selective mGluR agonist (R,S)-4-phosphonophenylglycine (PPG). Consistent with the lack of signs of epileptic activity in the absence of specific stimuli, mGluR7 -/- mice showed no major changes in synaptic properties in two slice preparations. However, slightly increased excitability was evident in hippocampal slices. In addition, there was slower recovery from frequency facilitation in cortical slices, suggesting a role for mGluR7 as a frequency-dependent regulator in presynaptic terminals. Our findings suggest that mGluR7 receptors have a unique role in regulating neuronal excitability and that these receptors may be a novel target for the development of anticonvulsant drugs. Society for Neuroscience2001 eng 0270-6474 1169858510.1523/JNEUROSCI.21-22-08734.2001 21228734 - 8745 yes Sansig G, Bushell T, Clarke V, et al. Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. <i>Journal of Neuroscience</i>. 2001;21(22):8734-8745. doi:<a href="https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001">10.1523/JNEUROSCI.21-22-08734.2001</a> Sansig, Gilles, et al. “Increased Seizure Susceptibility in Mice Lacking Metabotropic Glutamate Receptor 7.” <i>Journal of Neuroscience</i>, vol. 21, no. 22, Society for Neuroscience, 2001, pp. 8734–45, doi:<a href="https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001">10.1523/JNEUROSCI.21-22-08734.2001</a>. Sansig G, Bushell T, Clarke V, Rozov A, Burnashev N, Portet C, Gasparini F, Schmutz M, Klebs K, Shigemoto R, Flor P, Kühn R, Knoepfel T, Schroeder M, Hampson D, Collett V, Zhang C, Duvoisin R, Collingridge G, Van Der Putten H. 2001. Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. Journal of Neuroscience. 21(22), 8734–8745. G. Sansig <i>et al.</i>, “Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7,” <i>Journal of Neuroscience</i>, vol. 21, no. 22. Society for Neuroscience, pp. 8734–8745, 2001. Sansig, Gilles, Trevor Bushell, Vernon Clarke, Andrei Rozov, Nail Burnashev, Chantal Portet, Fabrizio Gasparini, et al. “Increased Seizure Susceptibility in Mice Lacking Metabotropic Glutamate Receptor 7.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2001. <a href="https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001">https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001</a>. G. Sansig, T. Bushell, V. Clarke, A. Rozov, N. Burnashev, C. Portet, F. Gasparini, M. Schmutz, K. Klebs, R. Shigemoto, P. Flor, R. Kühn, T. Knoepfel, M. Schroeder, D. Hampson, V. Collett, C. Zhang, R. Duvoisin, G. Collingridge, H. Van Der Putten, Journal of Neuroscience 21 (2001) 8734–8745. Sansig, G., Bushell, T., Clarke, V., Rozov, A., Burnashev, N., Portet, C., … Van Der Putten, H. (2001). Increased seizure susceptibility in mice lacking metabotropic glutamate receptor 7. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href="https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001">https://doi.org/10.1523/JNEUROSCI.21-22-08734.2001</a> 26102018-12-11T11:58:39Z2023-05-24T08:47:53Z