---
res:
  bibo_abstract:
  - In this investigation, we report identification and characterization of a 95 kDa
    postsynaptic density protein (PSD-95)/discs-large/ ZO-1 (PDZ) domain-containing
    protein termed tamalin, also recently named GRP1-associated scaffold protein (GRASP),
    that interacts with group 1 metabotropic glutamate receptors (mGluRs). The yeast
    two-hybrid system and in vitro pull-down assays indicated that the PDZ domain-containing,
    amino-terminal half of tamalin directly binds to the class I PDZ-binding motif
    of group 1 mGluRs. The C-terminal half of tamalin also bound to cytohesins, the
    members of guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation
    factor (ARF) family of small GTP-binding proteins. Tamalin mRNA is expressed predominantly
    in the telencephalic region and highly overlaps with the expression of group 1
    mGluR mRNAs. Both tamalin and cytohesin-2 were enriched and codistributed with
    mGluR1a in postsynaptic membrane fractions. Importantly, recombinant and native
    mGluR1a/tamalin/cytohesin-2 complexes were coimmunoprecipitated from transfected
    COS-7 cells and rat brain tissue, respectively. Transfection of tamalin and mutant
    tamalin lacking a cytohesin-binding domain caused an increase and decrease in
    cell-surface expression of mGluR1a in COS-7 cells, respectively. Furthermore,
    adenovirus-mediated expression of tamalin and dominant-negative tamalin facilitated
    and reduced the neuritic distribution of endogenous mGluR5 in cultured hippocampal
    neurons, respectively. The results indicate that tamalin plays a key role in the
    association of group 1 mGluRs with the ARF-specific GEF proteins and contributes
    to intracellular trafficking and the macromolecular organization of group 1 mGluRs
    at synapses.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jun
      foaf_name: Kitano, Jun
      foaf_surname: Kitano
  - foaf_Person:
      foaf_givenName: Kouji
      foaf_name: Kimura, Kouji
      foaf_surname: Kimura
  - foaf_Person:
      foaf_givenName: Yoshimitsu
      foaf_name: Yamazaki, Yoshimitsu
      foaf_surname: Yamazaki
  - foaf_Person:
      foaf_givenName: Takeshi
      foaf_name: Soda, Takeshi
      foaf_surname: Soda
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  - foaf_Person:
      foaf_givenName: Yoshiaki
      foaf_name: Nakajima, Yoshiaki
      foaf_surname: Nakajima
  - foaf_Person:
      foaf_givenName: Shigetada
      foaf_name: Nakanishi, Shigetada
      foaf_surname: Nakanishi
  bibo_doi: 10.1523/JNEUROSCI.22-04-01280.2002
  bibo_issue: '4'
  bibo_volume: 22
  dct_date: 2002^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0270-6474
  dct_language: eng
  dct_publisher: Society for Neuroscience@
  dct_title: Tamalin, a PDZ domain-containing protein, links a protein complex formation
    of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange
    factor cytohesins@
  fabio_hasPubmedId: '11850456'
...