---
_id: '2619'
abstract:
- lang: eng
text: The release of glutamate and GABA is modulated by presynaptic metabotropic
glutamate receptors (mGluRs). We used immunocytochemical methods to define the
location of the group III receptor mGluR7a in glutamatergic and GABAergic terminals
innervating GABAergic interneurons and pyramidal cells. Immunoreactivity for mGluR7a
was localized in the presynaptic active zone of both identified GABAergic and
presumed glutamatergic terminals. Terminals innervating dendritic spines showed
a variable level of receptor immunoreactivity, ranging from immunonegative to
strongly immunopositive. The frequency of strongly mGluR7a positive terminals
innervating the soma and dendrites of mGluR1α/somatostatin-expressing interneurons
was very high relative to other neurons. On dendrites that received mGluR7a-enriched
glutamatergic innervation, at least 80% of GABAergic terminals were immunopositive
for mGluR7a. On such dendrites virtually all (95%) vasoactive intestinal polypeptide
(VIP) positive (GABAergic) terminals were enriched in mGluR7a. The targets of
VIP/mGluR7a-expressing terminals were mainly (88%) mGluR1α-expressing interneurons,
which were mostly somatostatin immunopositive. Parvalbumin positive terminals
were immunonegative for mGluR7a. Some parvalbumin immunoreactive dendrites received
strongly mGluR7a positive terminals. The subcellular location, as well as the
cell type and synapse-specific distribution of mGluR7a in isocortical neuronal
circuits, is homologous to its distribution in the hippocampus. The specific location
of mGluR7a in the presynaptic active zone of both glutamatergic and GABAergic
synapses may be related to the proximity of calcium channels and the vesicle fusion
machinery. The enrichment of mGluR7a in the main GABAergic, as well as in the
glutamatergic, innervation of mGluR1α/somatostatin-expressing interneurons suggests
that their activation is under unique regulation by extracellular glutamate.
acknowledgement: We thank Dr C. Paspalas for an initial contribution to the immunocytochemistry.
We are grateful for the generous gifts of antibodies from Dr A. Buchan (anti-somatostatin,
Department of Physiology, University of British Columbia, Canada), Dr M. Watanabe
(anti-mGluR1α, Department of Anatomy, Hokkaido University School of Medicine, Sapporo)
and Dr K. Tanaka (anti-GAD, Niigata University, Faculty of Medicine, Department
of Neurology). We thank Dr F. Ferraguti for helpful suggestions during the project
and for his comments on a previous version of the manuscript. We also thank Philip
Cobden, Paul Jays and Laszlo Marton for assistance. Y.D. was supported by a Wellcome
Trust Advanced Training Fellowship.
article_processing_charge: No
article_type: original
author:
- first_name: Yannis
full_name: Dalezios, Yannis
last_name: Dalezios
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: John
full_name: Roberts, John
last_name: Roberts
- first_name: Péter
full_name: Somogyi, Péter
last_name: Somogyi
citation:
ama: Dalezios Y, Luján R, Shigemoto R, Roberts J, Somogyi P. Enrichment of mGluR7a
in the Presynaptic active zones of GABAergic and Non-GABAergic terminals on interneurons
in the rat somatosensory cortex. Cerebral Cortex. 2002;12(9):961-974. doi:10.1093/cercor/12.9.961
apa: Dalezios, Y., Luján, R., Shigemoto, R., Roberts, J., & Somogyi, P. (2002).
Enrichment of mGluR7a in the Presynaptic active zones of GABAergic and Non-GABAergic
terminals on interneurons in the rat somatosensory cortex. Cerebral Cortex.
Oxford University Press. https://doi.org/10.1093/cercor/12.9.961
chicago: Dalezios, Yannis, Rafael Luján, Ryuichi Shigemoto, John Roberts, and Péter
Somogyi. “Enrichment of MGluR7a in the Presynaptic Active Zones of GABAergic and
Non-GABAergic Terminals on Interneurons in the Rat Somatosensory Cortex.” Cerebral
Cortex. Oxford University Press, 2002. https://doi.org/10.1093/cercor/12.9.961.
ieee: Y. Dalezios, R. Luján, R. Shigemoto, J. Roberts, and P. Somogyi, “Enrichment
of mGluR7a in the Presynaptic active zones of GABAergic and Non-GABAergic terminals
on interneurons in the rat somatosensory cortex,” Cerebral Cortex, vol.
12, no. 9. Oxford University Press, pp. 961–974, 2002.
ista: Dalezios Y, Luján R, Shigemoto R, Roberts J, Somogyi P. 2002. Enrichment of
mGluR7a in the Presynaptic active zones of GABAergic and Non-GABAergic terminals
on interneurons in the rat somatosensory cortex. Cerebral Cortex. 12(9), 961–974.
mla: Dalezios, Yannis, et al. “Enrichment of MGluR7a in the Presynaptic Active Zones
of GABAergic and Non-GABAergic Terminals on Interneurons in the Rat Somatosensory
Cortex.” Cerebral Cortex, vol. 12, no. 9, Oxford University Press, 2002,
pp. 961–74, doi:10.1093/cercor/12.9.961.
short: Y. Dalezios, R. Luján, R. Shigemoto, J. Roberts, P. Somogyi, Cerebral Cortex
12 (2002) 961–974.
date_created: 2018-12-11T11:58:42Z
date_published: 2002-09-01T00:00:00Z
date_updated: 2023-07-25T09:40:49Z
day: '01'
doi: 10.1093/cercor/12.9.961
extern: '1'
external_id:
pmid:
- '12183395'
intvolume: ' 12'
issue: '9'
language:
- iso: eng
month: '09'
oa_version: None
page: 961 - 974
pmid: 1
publication: Cerebral Cortex
publication_identifier:
issn:
- 1047-3211
publication_status: published
publisher: Oxford University Press
publist_id: '4280'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Enrichment of mGluR7a in the Presynaptic active zones of GABAergic and Non-GABAergic
terminals on interneurons in the rat somatosensory cortex
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 12
year: '2002'
...