---
_id: '2620'
abstract:
- lang: eng
  text: An ion channel's function depends largely on its location and density on neurons.
    Here we used high-resolution immunolocalization to determine the subcellular distribution
    of the hyperpolarization-activated and cyclic-nucleotide-gated channel subunit
    1 (HCN1) in rat brain. Light microscopy revealed graded HCN1 immunoreactivity
    in apical dendrites of hippocampal, subicular and neocortical layer-5 pyramidal
    cells. Quantitative comparison of immunogold densities showed a 60-fold increase
    from somatic to distal apical dendritic membranes. Distal dendritic shafts had
    16 times more HCN1 labeling than proximal dendrites of similar diameters. At the
    same distance from the soma, the density of HCN1 was significantly higher in dendritic
    shafts than in spines. Our results reveal the complex cell surface distribution
    of voltage-gated ion-channels, and predict its role in increasing the computational
    power of single neurons via subcellular domain and input-specific mechanisms.
acknowledgement: Z.N. received grants from the Hungarian Science Foundation (T032309),
  the Howard Hughes Medical Institute, the James S. McDonnell Foundation, the Wellcome
  Trust and the Boehringer Ingelheim Fund. Z.N. and R.S. received grants from CREST—Japan
  Science and Technology Corporation. G.T. is funded by the Wellcome Trust.
article_processing_charge: No
article_type: original
author:
- first_name: Andrea
  full_name: Lörincz, Andrea
  last_name: Lörincz
- first_name: Takuya
  full_name: Notomi, Takuya
  last_name: Notomi
- first_name: Gábor
  full_name: Tamás, Gábor
  last_name: Tamás
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Zoltán
  full_name: Nusser, Zoltán
  last_name: Nusser
citation:
  ama: Lörincz A, Notomi T, Tamás G, Shigemoto R, Nusser Z. Polarized and compartment-dependent
    distribution of HCN1 in pyramidal cell dendrites. <i>Nature Neuroscience</i>.
    2002;5(11):1185-1193. doi:<a href="https://doi.org/10.1038/nn962">10.1038/nn962</a>
  apa: Lörincz, A., Notomi, T., Tamás, G., Shigemoto, R., &#38; Nusser, Z. (2002).
    Polarized and compartment-dependent distribution of HCN1 in pyramidal cell dendrites.
    <i>Nature Neuroscience</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nn962">https://doi.org/10.1038/nn962</a>
  chicago: Lörincz, Andrea, Takuya Notomi, Gábor Tamás, Ryuichi Shigemoto, and Zoltán
    Nusser. “Polarized and Compartment-Dependent Distribution of HCN1 in Pyramidal
    Cell Dendrites.” <i>Nature Neuroscience</i>. Nature Publishing Group, 2002. <a
    href="https://doi.org/10.1038/nn962">https://doi.org/10.1038/nn962</a>.
  ieee: A. Lörincz, T. Notomi, G. Tamás, R. Shigemoto, and Z. Nusser, “Polarized and
    compartment-dependent distribution of HCN1 in pyramidal cell dendrites,” <i>Nature
    Neuroscience</i>, vol. 5, no. 11. Nature Publishing Group, pp. 1185–1193, 2002.
  ista: Lörincz A, Notomi T, Tamás G, Shigemoto R, Nusser Z. 2002. Polarized and compartment-dependent
    distribution of HCN1 in pyramidal cell dendrites. Nature Neuroscience. 5(11),
    1185–1193.
  mla: Lörincz, Andrea, et al. “Polarized and Compartment-Dependent Distribution of
    HCN1 in Pyramidal Cell Dendrites.” <i>Nature Neuroscience</i>, vol. 5, no. 11,
    Nature Publishing Group, 2002, pp. 1185–93, doi:<a href="https://doi.org/10.1038/nn962">10.1038/nn962</a>.
  short: A. Lörincz, T. Notomi, G. Tamás, R. Shigemoto, Z. Nusser, Nature Neuroscience
    5 (2002) 1185–1193.
date_created: 2018-12-11T11:58:43Z
date_published: 2002-11-01T00:00:00Z
date_updated: 2023-07-25T09:02:48Z
day: '01'
doi: 10.1038/nn962
extern: '1'
external_id:
  pmid:
  - '12389030'
intvolume: '         5'
issue: '11'
language:
- iso: eng
month: '11'
oa_version: None
page: 1185 - 1193
pmid: 1
publication: Nature Neuroscience
publication_identifier:
  issn:
  - 1097-6256
publication_status: published
publisher: Nature Publishing Group
publist_id: '4278'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Polarized and compartment-dependent distribution of HCN1 in pyramidal cell
  dendrites
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 5
year: '2002'
...