{"type":"journal_article","citation":{"ieee":"A. Momiyama <i>et al.</i>, “The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats,” <i>Journal of Physiology</i>, vol. 549, no. 1. Wiley-Blackwell, pp. 75–92, 2003.","ama":"Momiyama A, Silver R, Häusser M, et al. The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. <i>Journal of Physiology</i>. 2003;549(1):75-92. doi:<a href=\"https://doi.org/10.1113/jphysiol.2002.033472\">10.1113/jphysiol.2002.033472</a>","ista":"Momiyama A, Silver R, Häusser M, Notomi T, Wu Y, Shigemoto R, Cull Candy S. 2003. The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. Journal of Physiology. 549(1), 75–92.","short":"A. Momiyama, R. Silver, M. Häusser, T. Notomi, Y. Wu, R. Shigemoto, S. Cull Candy, Journal of Physiology 549 (2003) 75–92.","mla":"Momiyama, Akiko, et al. “The Density of AMPA Receptors Activated by a Transmitter Quantum at the Climbing Fibre - Purkinje Cell Synapse in Immature Rats.” <i>Journal of Physiology</i>, vol. 549, no. 1, Wiley-Blackwell, 2003, pp. 75–92, doi:<a href=\"https://doi.org/10.1113/jphysiol.2002.033472\">10.1113/jphysiol.2002.033472</a>.","chicago":"Momiyama, Akiko, Rachel Silver, Michael Häusser, Takuya Notomi, Yue Wu, Ryuichi Shigemoto, and Stuart Cull Candy. “The Density of AMPA Receptors Activated by a Transmitter Quantum at the Climbing Fibre - Purkinje Cell Synapse in Immature Rats.” <i>Journal of Physiology</i>. Wiley-Blackwell, 2003. <a href=\"https://doi.org/10.1113/jphysiol.2002.033472\">https://doi.org/10.1113/jphysiol.2002.033472</a>.","apa":"Momiyama, A., Silver, R., Häusser, M., Notomi, T., Wu, Y., Shigemoto, R., &#38; Cull Candy, S. (2003). The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats. <i>Journal of Physiology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1113/jphysiol.2002.033472\">https://doi.org/10.1113/jphysiol.2002.033472</a>"},"publication_status":"published","day":"15","date_created":"2018-12-11T11:58:45Z","publication":"Journal of Physiology","date_published":"2003-05-15T00:00:00Z","_id":"2628","volume":549,"date_updated":"2021-01-12T06:58:40Z","author":[{"first_name":"Akiko","full_name":"Momiyama, Akiko","last_name":"Momiyama"},{"first_name":"Rachel","last_name":"Silver","full_name":"Silver, Rachel A"},{"last_name":"Häusser","full_name":"Häusser, Michael A","first_name":"Michael"},{"last_name":"Notomi","full_name":"Notomi, Takuya","first_name":"Takuya"},{"full_name":"Wu, Yue","last_name":"Wu","first_name":"Yue"},{"first_name":"Ryuichi","full_name":"Ryuichi Shigemoto","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444"},{"full_name":"Cull-Candy, Stuart G","last_name":"Cull Candy","first_name":"Stuart"}],"month":"05","doi":"10.1113/jphysiol.2002.033472","title":"The density of AMPA receptors activated by a transmitter quantum at the climbing fibre - Purkinje cell synapse in immature rats","abstract":[{"lang":"eng","text":"We aimed to estimate the number of AMPA receptors (AMPARs) bound by the quantal transmitter packet, their single-channel conductance and their density in the postsynaptic membrane at cerebellar Purkinje cell synapses. The synaptic and extrasynaptic AMPARs were examined in Purkinje cells in 2- to 4-day-old rats, when they receive synaptic inputs solely from climbing fibres (CFs). Evoked CF EPSCs and whole-cell AMPA currents displayed roughly linear current-voltage relationships, consistent with the presence of GluR2 subunits in synaptic and extrasynaptic AMPARs. The mean quantal size, estimated from the miniature EPSCs (MEPSCs), was ∼300 pS. Peak-scaled non-stationary fluctuation analysis of spontaneous EPSCs and MEPSCs gave a weighted-mean synaptic channel conductance of ∼5 pS (∼7 pS when corrected for filtering). By applying non-stationary fluctuation analysis to extrasynaptic currents activated by brief glutamate pulses (5 mM), we also obtained a small single-channel conductance estimate for extrasynaptic AMPARs (∼11 pS). This approach allowed us to obtain a maximum open probability (Po,max) value for the extrasynaptic receptors (Po,max = 0.72). Directly resolved extrasynaptic channel openings in the continued presence of glutamate exhibited clear multiple-conductance levels. The mean area of the postsynaptic density (PSD) of these synapses was 0.074 μm2, measured by reconstructing electron-microscopic (EM) serial sections. Postembedding immunogold labelling by anti-GluR2/3 antibody revealed that AMPARs are localised in PSDs. From these data and by simulating error factors, we estimate that at least 66 AMPARs are bound by a quantal transmitter packet at CF-Purkinje cell synapses, and the receptors are packed at a minimum density of ∼900 μm-2 in the postsynaptic membrane."}],"year":"2003","publist_id":"4270","issue":"1","quality_controlled":0,"publisher":"Wiley-Blackwell","intvolume":"       549","status":"public","extern":1,"page":"75 - 92"}