---
_id: '2635'
abstract:
- lang: eng
text: Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically
and postsynaptically. Using preembedding immunohistochemical methods combined
with quantitative analysis of GABAB receptor subunit immunoreactivity, this study
provides a detailed description of the cellular and subcellular localization of
GABAB1a/b and GABA B2 in the rat hippocampus. At the light microscopic level,
an overlapping distribution of GABAB1a/b and GABAB2 was revealed in the dendritic
layers of the hippocampus. In addition, expression of the GABAB1a/b subunit was
found in somata of CA1 pyramidal cells and of a subset of GABAergic interneurons.
At the electron microscopic level, immunoreactivity for both subunits was observed
on presynaptic and, more abundantly, on postsynaptic elements. Presynaptically,
subunits were mainly detected in the extrasynaptic membrane and occasionally over
the presynaptic membrane specialization of putative glutamatergic and, to a lesser
extent, GABAergic axon terminals. Postsynaptically, the majority of GABAB receptor
subunits were localized to the extrasynaptic plasma membrane of spines and dendritic
shafts of principal cells and shafts of interneuron dendrites. Quantitative analysis
revealed enrichment of GABAB1a/b around putative glutamatergic synapses on spines
and an even distribution on dendritic shafts of pyramidal cells contacted by GABAergic
boutons. The association of GABAB receptors with glutamatergic synapses at both
presynaptic and postsynaptic sides indicates their intimate involvement in the
modulation of glutamatergic neurotransmission. The dominant extrasynaptic localization
of GABAB receptor subunits suggests that their activation is dependent on spillover
of GABA requiring simultaneous activity of populations of GABAergic cells as it
occurs during population oscillations or epileptic seizures.
author:
- first_name: Ákos
full_name: Kulik, Ákos
last_name: Kulik
- first_name: Imre
full_name: Vida, Imre
last_name: Vida
- first_name: Rafael
full_name: Luján, Rafael
last_name: Luján
- first_name: Carola
full_name: Haas, Carola A
last_name: Haas
- first_name: Guillermina
full_name: López-Bendito, Guillermina
last_name: López Bendito
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
citation:
ama: Kulik Á, Vida I, Luján R, et al. Subcellular Localization of Metabotropic GABAB
Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience.
2003;23(35):11026-11035.
apa: Kulik, Á., Vida, I., Luján, R., Haas, C., López Bendito, G., Shigemoto, R.,
& Frotscher, M. (2003). Subcellular Localization of Metabotropic GABAB Receptor
Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience.
Society for Neuroscience.
chicago: Kulik, Ákos, Imre Vida, Rafael Luján, Carola Haas, Guillermina López Bendito,
Ryuichi Shigemoto, and Michael Frotscher. “Subcellular Localization of Metabotropic
GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal
of Neuroscience. Society for Neuroscience, 2003.
ieee: Á. Kulik et al., “Subcellular Localization of Metabotropic GABAB Receptor
Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus,” Journal of Neuroscience,
vol. 23, no. 35. Society for Neuroscience, pp. 11026–11035, 2003.
ista: Kulik Á, Vida I, Luján R, Haas C, López Bendito G, Shigemoto R, Frotscher
M. 2003. Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b
and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. 23(35), 11026–11035.
mla: Kulik, Ákos, et al. “Subcellular Localization of Metabotropic GABAB Receptor
Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal of Neuroscience,
vol. 23, no. 35, Society for Neuroscience, 2003, pp. 11026–35.
short: Á. Kulik, I. Vida, R. Luján, C. Haas, G. López Bendito, R. Shigemoto, M.
Frotscher, Journal of Neuroscience 23 (2003) 11026–11035.
date_created: 2018-12-11T11:58:47Z
date_published: 2003-12-03T00:00:00Z
date_updated: 2021-01-12T06:58:43Z
day: '03'
extern: 1
intvolume: ' 23'
issue: '35'
month: '12'
page: 11026 - 11035
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '4263'
quality_controlled: 0
status: public
title: Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b
and GABAB2 in the Rat Hippocampus
type: journal_article
volume: 23
year: '2003'
...