--- _id: '2635' abstract: - lang: eng text: Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and postsynaptically. Using preembedding immunohistochemical methods combined with quantitative analysis of GABAB receptor subunit immunoreactivity, this study provides a detailed description of the cellular and subcellular localization of GABAB1a/b and GABA B2 in the rat hippocampus. At the light microscopic level, an overlapping distribution of GABAB1a/b and GABAB2 was revealed in the dendritic layers of the hippocampus. In addition, expression of the GABAB1a/b subunit was found in somata of CA1 pyramidal cells and of a subset of GABAergic interneurons. At the electron microscopic level, immunoreactivity for both subunits was observed on presynaptic and, more abundantly, on postsynaptic elements. Presynaptically, subunits were mainly detected in the extrasynaptic membrane and occasionally over the presynaptic membrane specialization of putative glutamatergic and, to a lesser extent, GABAergic axon terminals. Postsynaptically, the majority of GABAB receptor subunits were localized to the extrasynaptic plasma membrane of spines and dendritic shafts of principal cells and shafts of interneuron dendrites. Quantitative analysis revealed enrichment of GABAB1a/b around putative glutamatergic synapses on spines and an even distribution on dendritic shafts of pyramidal cells contacted by GABAergic boutons. The association of GABAB receptors with glutamatergic synapses at both presynaptic and postsynaptic sides indicates their intimate involvement in the modulation of glutamatergic neurotransmission. The dominant extrasynaptic localization of GABAB receptor subunits suggests that their activation is dependent on spillover of GABA requiring simultaneous activity of populations of GABAergic cells as it occurs during population oscillations or epileptic seizures. author: - first_name: Ákos full_name: Kulik, Ákos last_name: Kulik - first_name: Imre full_name: Vida, Imre last_name: Vida - first_name: Rafael full_name: Luján, Rafael last_name: Luján - first_name: Carola full_name: Haas, Carola A last_name: Haas - first_name: Guillermina full_name: López-Bendito, Guillermina last_name: López Bendito - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: Michael full_name: Frotscher, Michael last_name: Frotscher citation: ama: Kulik Á, Vida I, Luján R, et al. Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. 2003;23(35):11026-11035. apa: Kulik, Á., Vida, I., Luján, R., Haas, C., López Bendito, G., Shigemoto, R., & Frotscher, M. (2003). Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. Society for Neuroscience. chicago: Kulik, Ákos, Imre Vida, Rafael Luján, Carola Haas, Guillermina López Bendito, Ryuichi Shigemoto, and Michael Frotscher. “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal of Neuroscience. Society for Neuroscience, 2003. ieee: Á. Kulik et al., “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus,” Journal of Neuroscience, vol. 23, no. 35. Society for Neuroscience, pp. 11026–11035, 2003. ista: Kulik Á, Vida I, Luján R, Haas C, López Bendito G, Shigemoto R, Frotscher M. 2003. Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus. Journal of Neuroscience. 23(35), 11026–11035. mla: Kulik, Ákos, et al. “Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus.” Journal of Neuroscience, vol. 23, no. 35, Society for Neuroscience, 2003, pp. 11026–35. short: Á. Kulik, I. Vida, R. Luján, C. Haas, G. López Bendito, R. Shigemoto, M. Frotscher, Journal of Neuroscience 23 (2003) 11026–11035. date_created: 2018-12-11T11:58:47Z date_published: 2003-12-03T00:00:00Z date_updated: 2021-01-12T06:58:43Z day: '03' extern: 1 intvolume: ' 23' issue: '35' month: '12' page: 11026 - 11035 publication: Journal of Neuroscience publication_status: published publisher: Society for Neuroscience publist_id: '4263' quality_controlled: 0 status: public title: Subcellular Localization of Metabotropic GABAB Receptor Subunits GABAB1a/b and GABAB2 in the Rat Hippocampus type: journal_article volume: 23 year: '2003' ...