--- res: bibo_abstract: - Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified > 1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Markus foaf_name: Brown, Markus foaf_surname: Brown foaf_workInfoHomepage: http://www.librecat.org/personId=3DAB9AFC-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Louise foaf_name: Johnson, Louise foaf_surname: Johnson - foaf_Person: foaf_givenName: Dario foaf_name: Leone, Dario foaf_surname: Leone - foaf_Person: foaf_givenName: Peter foaf_name: Májek, Peter foaf_surname: Májek - foaf_Person: foaf_givenName: Kari foaf_name: Vaahtomeri, Kari foaf_surname: Vaahtomeri foaf_workInfoHomepage: http://www.librecat.org/personId=368EE576-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-7829-3518 - foaf_Person: foaf_givenName: Daniel foaf_name: Senfter, Daniel foaf_surname: Senfter - foaf_Person: foaf_givenName: Nora foaf_name: Bukosza, Nora foaf_surname: Bukosza - foaf_Person: foaf_givenName: Helga foaf_name: Schachner, Helga foaf_surname: Schachner - foaf_Person: foaf_givenName: Gabriele foaf_name: Asfour, Gabriele foaf_surname: Asfour - foaf_Person: foaf_givenName: Brigitte foaf_name: Langer, Brigitte foaf_surname: Langer - foaf_Person: foaf_givenName: Robert foaf_name: Hauschild, Robert foaf_surname: Hauschild foaf_workInfoHomepage: http://www.librecat.org/personId=4E01D6B4-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9843-3522 - foaf_Person: foaf_givenName: Katja foaf_name: Parapatics, Katja foaf_surname: Parapatics - foaf_Person: foaf_givenName: Young foaf_name: Hong, Young foaf_surname: Hong - foaf_Person: foaf_givenName: Keiryn foaf_name: Bennett, Keiryn foaf_surname: Bennett - foaf_Person: foaf_givenName: Renate foaf_name: Kain, Renate foaf_surname: Kain - foaf_Person: foaf_givenName: Michael foaf_name: Detmar, Michael foaf_surname: Detmar - foaf_Person: foaf_givenName: Michael K foaf_name: Sixt, Michael K foaf_surname: Sixt foaf_workInfoHomepage: http://www.librecat.org/personId=41E9FBEA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6620-9179 - foaf_Person: foaf_givenName: David foaf_name: Jackson, David foaf_surname: Jackson - foaf_Person: foaf_givenName: Dontscho foaf_name: Kerjaschki, Dontscho foaf_surname: Kerjaschki bibo_doi: 10.1083/jcb.201612051 bibo_issue: '6' bibo_volume: 217 dct_date: 2018^xs_gYear dct_identifier: - UT:000438077800026 dct_language: eng dct_publisher: Rockefeller University Press@ dct_title: Lymphatic exosomes promote dendritic cell migration along guidance cues@ fabio_hasPubmedId: '29650776' ...