---
res:
  bibo_abstract:
  - Directed migration of cells relies on their ability to sense directional guidance
    cues and to interact with pericellular structures in order to transduce contractile
    cytoskeletal- into mechanical forces. These biomechanical processes depend highly
    on microenvironmental factors such as exposure to 2D surfaces or 3D matrices.
    In vivo, the majority of cells are exposed to 3D environments. Data on 3D cell
    migration are mostly derived from intravital microscopy or collagen-based in vitro
    assays. Both approaches offer only limited controlla-bility of experimental conditions.
    Here, we developed an automated microfluidic system that allows positioning of
    cells in 3D microenvironments containing highly controlled diffusion-based chemokine
    gradients. Tracking migration in such gradients was feasible in real time at the
    single cell level. Moreover, the setup allowed on-chip immunocytochemistry and
    thus linking of functional with phenotypical properties in individual cells. Spatially
    defined retrieval of cells from the device allows down-stream off-chip analysis.
    Using dendritic cells as a model, our setup specifically allowed us for the first
    time to quantitate key migration characteristics of cells exposed to identical
    gradients of the chemokine CCL19 yet placed on 2D vs in 3D environments. Migration
    properties between 2D and 3D migration were distinct. Morphological features of
    cells migrating in an in vitro 3D environment were similar to those of cells migrating
    in animal tissues, but different from cells migrating on a surface. Our system
    thus offers a highly controllable in vitro-mimic of a 3D environment that cells
    traffic in vivo.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Corina
      foaf_name: Frick, Corina
      foaf_surname: Frick
  - foaf_Person:
      foaf_givenName: Philip
      foaf_name: Dettinger, Philip
      foaf_surname: Dettinger
  - foaf_Person:
      foaf_givenName: Jörg
      foaf_name: Renkawitz, Jörg
      foaf_surname: Renkawitz
      foaf_workInfoHomepage: http://www.librecat.org/personId=3F0587C8-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-2856-3369
  - foaf_Person:
      foaf_givenName: Annaïse
      foaf_name: Jauch, Annaïse
      foaf_surname: Jauch
  - foaf_Person:
      foaf_givenName: Christoph
      foaf_name: Berger, Christoph
      foaf_surname: Berger
  - foaf_Person:
      foaf_givenName: Mike
      foaf_name: Recher, Mike
      foaf_surname: Recher
  - foaf_Person:
      foaf_givenName: Timm
      foaf_name: Schroeder, Timm
      foaf_surname: Schroeder
  - foaf_Person:
      foaf_givenName: Matthias
      foaf_name: Mehling, Matthias
      foaf_surname: Mehling
  bibo_doi: 10.1371/journal.pone.0198330
  bibo_issue: '6'
  bibo_volume: 13
  dct_date: 2018^xs_gYear
  dct_identifier:
  - UT:000434384900031
  dct_language: eng
  dct_publisher: Public Library of Science@
  dct_title: Nano-scale microfluidics to study 3D chemotaxis at the single cell level@
...