---
res:
  bibo_abstract:
  - Light represents the principal signal driving circadian clock entrainment. However,
    how light influences the evolution of the clock remains poorly understood. The
    cavefish Phreatichthys andruzzii represents a fascinating model to explore how
    evolution under extreme aphotic conditions shapes the circadian clock, since in
    this species the clock is unresponsive to light. We have previously demonstrated
    that loss-of-function mutations targeting non-visual opsins contribute in part
    to this blind clock phenotype. Here, we have compared orthologs of two core clock
    genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish
    and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii
    per2 transcript. The most abundant transcript encodes a truncated protein lacking
    the C-terminal Cry binding domain and incorporating an intronic, transposon-derived
    coding sequence. We demonstrate that the transposon insertion leads to a predominantly
    cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish
    ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems
    that during evolution in complete darkness, the photic entrainment pathway of
    the circadian clock has been subject to mutation at multiple levels, extending
    from opsin photoreceptors to nuclear effectors.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Rosa Maria
      foaf_name: Ceinos, Rosa Maria
      foaf_surname: Ceinos
  - foaf_Person:
      foaf_givenName: Elena
      foaf_name: Frigato, Elena
      foaf_surname: Frigato
  - foaf_Person:
      foaf_givenName: Cristina
      foaf_name: Pagano, Cristina
      foaf_surname: Pagano
  - foaf_Person:
      foaf_givenName: Nadine
      foaf_name: Frohlich, Nadine
      foaf_surname: Frohlich
  - foaf_Person:
      foaf_givenName: Pietro
      foaf_name: Negrini, Pietro
      foaf_surname: Negrini
  - foaf_Person:
      foaf_givenName: Nicola
      foaf_name: Cavallari, Nicola
      foaf_surname: Cavallari
      foaf_workInfoHomepage: http://www.librecat.org/personId=457160E6-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Daniela
      foaf_name: Vallone, Daniela
      foaf_surname: Vallone
  - foaf_Person:
      foaf_givenName: Silvia
      foaf_name: Fuselli, Silvia
      foaf_surname: Fuselli
  - foaf_Person:
      foaf_givenName: Cristiano
      foaf_name: Bertolucci, Cristiano
      foaf_surname: Bertolucci
  - foaf_Person:
      foaf_givenName: Nicholas S
      foaf_name: Foulkes, Nicholas S
      foaf_surname: Foulkes
  bibo_doi: 10.1038/s41598-018-27080-2
  bibo_issue: '1'
  bibo_volume: 8
  dct_date: 2018^xs_gYear
  dct_identifier:
  - UT:000434640800008
  dct_language: eng
  dct_publisher: Nature Publishing Group@
  dct_title: Mutations in blind cavefish target the light regulated circadian clock
    gene period 2@
...