{"intvolume":"       998","month":"02","language":[{"iso":"eng"}],"ddc":["570"],"year":"2013","publication":"Methods in Molecular Biology","date_updated":"2021-01-12T07:00:17Z","project":[{"grant_number":"RGY0084/2012","name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)","_id":"255BFFFA-B435-11E9-9278-68D0E5697425"},{"_id":"25548C20-B435-11E9-9278-68D0E5697425","name":"Microbial Ion Channels for Synthetic Neurobiology","grant_number":"303564","call_identifier":"FP7"}],"citation":{"ista":"Szobota S, Mckenzie C, Janovjak HL. 2013. Optical control of ligand-gated ion channels. Methods in Molecular Biology. 998, 417–435.","mla":"Szobota, Stephanie, et al. “Optical Control of Ligand-Gated Ion Channels.” <i>Methods in Molecular Biology</i>, vol. 998, Springer, 2013, pp. 417–35, doi:<a href=\"https://doi.org/10.1007/978-1-62703-351-0_32\">10.1007/978-1-62703-351-0_32</a>.","short":"S. Szobota, C. Mckenzie, H.L. Janovjak, Methods in Molecular Biology 998 (2013) 417–435.","ama":"Szobota S, Mckenzie C, Janovjak HL. Optical control of ligand-gated ion channels. <i>Methods in Molecular Biology</i>. 2013;998:417-435. doi:<a href=\"https://doi.org/10.1007/978-1-62703-351-0_32\">10.1007/978-1-62703-351-0_32</a>","apa":"Szobota, S., Mckenzie, C., &#38; Janovjak, H. L. (2013). Optical control of ligand-gated ion channels. <i>Methods in Molecular Biology</i>. Springer. <a href=\"https://doi.org/10.1007/978-1-62703-351-0_32\">https://doi.org/10.1007/978-1-62703-351-0_32</a>","chicago":"Szobota, Stephanie, Catherine Mckenzie, and Harald L Janovjak. “Optical Control of Ligand-Gated Ion Channels.” <i>Methods in Molecular Biology</i>. Springer, 2013. <a href=\"https://doi.org/10.1007/978-1-62703-351-0_32\">https://doi.org/10.1007/978-1-62703-351-0_32</a>.","ieee":"S. Szobota, C. Mckenzie, and H. L. Janovjak, “Optical control of ligand-gated ion channels,” <i>Methods in Molecular Biology</i>, vol. 998. Springer, pp. 417–435, 2013."},"file":[{"checksum":"1701f0d989f27ddac471b19a894ec0d1","date_created":"2018-12-12T10:12:34Z","file_id":"4952","content_type":"application/pdf","creator":"system","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:45:51Z","file_size":336734,"file_name":"IST-2017-834-v1+1_szobota.pdf"}],"abstract":[{"lang":"eng","text":"In the vibrant field of optogenetics, optics and genetic targeting are combined to commandeer cellular functions, such as the neuronal action potential, by optically stimulating light-sensitive ion channels expressed in the cell membrane. One broadly applicable manifestation of this approach are covalently attached photochromic tethered ligands (PTLs) that allow activating ligand-gated ion channels with outstanding spatial and temporal resolution. Here, we describe all steps towards the successful development and application of PTL-gated ion channels in cell lines and primary cells. The basis for these experiments forms a combination of molecular modeling, genetic engineering, cell culture, and electrophysiology. The light-gated glutamate receptor (LiGluR), which consists of the PTL-functionalized GluK2 receptor, serves as a model."}],"date_created":"2018-12-11T11:59:57Z","ec_funded":1,"type":"journal_article","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pubrep_id":"834","page":"417 - 435","publisher":"Springer","title":"Optical control of ligand-gated ion channels","publist_id":"3932","alternative_title":["MIMB"],"oa":1,"status":"public","has_accepted_license":"1","scopus_import":1,"date_published":"2013-02-22T00:00:00Z","day":"22","_id":"2857","department":[{"_id":"HaJa"}],"quality_controlled":"1","file_date_updated":"2020-07-14T12:45:51Z","volume":998,"oa_version":"Submitted Version","author":[{"first_name":"Stephanie","full_name":"Szobota, Stephanie","last_name":"Szobota"},{"last_name":"Mckenzie","full_name":"Mckenzie, Catherine","first_name":"Catherine","id":"3EEDE19A-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-8023-9315","first_name":"Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","full_name":"Janovjak, Harald L"}],"doi":"10.1007/978-1-62703-351-0_32"}