---
res:
  bibo_abstract:
  - Using an electrospray tandem mass spectrometer as a concentration-sensitive detector,
    a method has been developed to quantify femtomole amounts of plant growth regulators
    (i.e. isoprenoid type cytokinins, zeatin, dihydrozeatin, isopentenyladenine and
    their respective riboside and glucoside analogues) and the second messenger adenosine
    3':5'-cyclic monophosphate (3':5'-cAMP). Miniaturisation of the chromatographic
    setup using capillary high performance liquid chromatographic (HPLC) ion spray
    mass spectrometry increased the sensitivity to the low femtomole region. Application
    of automated capillary column switching allowed the introduction of large injection
    volumes into the HPLC system. Aliquots (25 μL) were injected into one dimension
    of the HPLC set-up and stacked onto a micro pre-column. By means of mobile phase
    switching the pre-column was back-flushed to introduce the analytes onto the analytical
    column. For cytokinin analysis positive electrospray ionisation was used and resulted
    in 2.5-25 fmol detection limits. Cyclic nucleotides were separated under ion-pair
    conditions using tetrabutyl ammonium bromide as ion-pair reagent and were detected
    under negative electrospray ionisation conditions. Here a 25 fmol detection limit
    was determined. Following this approach, cytokinins and 3':5'-cAMP extracted from
    only mg amounts of apical shoot meristem and chloroplasts obtained from Nicotiana
    tabacum cv. Petit Havana SR1 were identified and quantified.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Erwin
      foaf_name: Witters, Erwin
      foaf_surname: Witters
  - foaf_Person:
      foaf_givenName: Koen
      foaf_name: Vanhoutte, Koen
      foaf_surname: Vanhoutte
  - foaf_Person:
      foaf_givenName: Walter
      foaf_name: Dewitte, Walter
      foaf_surname: Dewitte
  - foaf_Person:
      foaf_givenName: Ivana
      foaf_name: Macháčková, Ivana
      foaf_surname: Macháčková
  - foaf_Person:
      foaf_givenName: Eva
      foaf_name: Benková, Eva
      foaf_surname: Benková
      foaf_workInfoHomepage: http://www.librecat.org/personId=38F4F166-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8510-9739
  - foaf_Person:
      foaf_givenName: Walter
      foaf_name: Van Dongen, Walter
      foaf_surname: Van Dongen
  - foaf_Person:
      foaf_givenName: Eddy
      foaf_name: Esmans, Eddy
      foaf_surname: Esmans
  - foaf_Person:
      foaf_givenName: Henri
      foaf_name: Van Onckelen, Henri
      foaf_surname: Van Onckelen
  bibo_doi: 10.1002/(SICI)1099-1565(199905/06)10:3<143::AID-PCA441>3.0.CO;2-G
  bibo_issue: '3'
  bibo_volume: 10
  dct_date: 1999^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0958-0344
  dct_language: eng
  dct_publisher: Wiley-Blackwell@
  dct_title: Analysis of cyclic nucleotides and cytokinins in minute plant samples
    using phase system switching capillary electrospray liquid chromatography tandem
    mass spectrometry@
...