---
_id: '2876'
abstract:
- lang: eng
text: Cytokinin (CK) activity is regulated by the complex interplay of their metabolism,
transport, stability and cellular/tissue localization. O-glucosides of zeatin-type
CKs are postulated to be storage and/or transport forms. Active CK levels are
determined in part by their differential distribution of CK metabolites across
different subcellular compartments. We have previously shown that overexpressing
chloroplast-localized Zm-p60.1, a maize β-glucosidase capable of releasing active
cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic
tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants
overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein
is correctly processed and localized to the vacuole. When grown on medium containing
exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to
ectopic growths at the base of the hypocotyl. The presence of the enzyme in these
ectopic structures is shown by histochemical staining. CK quantification reveals
that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to
levels similar to those observed in the wild type. When crossed with tobacco overexpressing
the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows
an almost complete reversion in the root elongation assay. This is the first evidence
from intact plants that the vacuole is the storage organelle for CK O-glucosides
and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1
as a robust molecular tool that exploits the reversibility of O-glucosylation
and enables delicate manipulations of active CK content at the cellular level.
author:
- first_name: Nagavalli
full_name: Kiran, Nagavalli S
last_name: Kiran
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Alena
full_name: Reková, Alena
last_name: Reková
- first_name: Jaroslava
full_name: Dubová, Jaroslava
last_name: Dubová
- first_name: Jiří
full_name: Malbeck, Jiří
last_name: Malbeck
- first_name: Klaus
full_name: Palme, Klaus
last_name: Palme
- first_name: Břetislav
full_name: Brzobohatý, Břetislav
last_name: Brzobohatý
citation:
ama: Kiran N, Benková E, Reková A, et al. Retargeting a maize β-glucosidase to the
vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the
vacuole. Phytochemistry. 2012;79:67-77. doi:10.1016/j.phytochem.2012.03.012
apa: Kiran, N., Benková, E., Reková, A., Dubová, J., Malbeck, J., Palme, K., &
Brzobohatý, B. (2012). Retargeting a maize β-glucosidase to the vacuole - Evidence
from intact plants that zeatin-O-glucoside is stored in the vacuole. Phytochemistry.
Elsevier. https://doi.org/10.1016/j.phytochem.2012.03.012
chicago: Kiran, Nagavalli, Eva Benková, Alena Reková, Jaroslava Dubová, Jiří Malbeck,
Klaus Palme, and Břetislav Brzobohatý. “Retargeting a Maize β-Glucosidase to the
Vacuole - Evidence from Intact Plants That Zeatin-O-Glucoside Is Stored in the
Vacuole.” Phytochemistry. Elsevier, 2012. https://doi.org/10.1016/j.phytochem.2012.03.012.
ieee: N. Kiran et al., “Retargeting a maize β-glucosidase to the vacuole
- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole,”
Phytochemistry, vol. 79. Elsevier, pp. 67–77, 2012.
ista: Kiran N, Benková E, Reková A, Dubová J, Malbeck J, Palme K, Brzobohatý B.
2012. Retargeting a maize β-glucosidase to the vacuole - Evidence from intact
plants that zeatin-O-glucoside is stored in the vacuole. Phytochemistry. 79, 67–77.
mla: Kiran, Nagavalli, et al. “Retargeting a Maize β-Glucosidase to the Vacuole
- Evidence from Intact Plants That Zeatin-O-Glucoside Is Stored in the Vacuole.”
Phytochemistry, vol. 79, Elsevier, 2012, pp. 67–77, doi:10.1016/j.phytochem.2012.03.012.
short: N. Kiran, E. Benková, A. Reková, J. Dubová, J. Malbeck, K. Palme, B. Brzobohatý,
Phytochemistry 79 (2012) 67–77.
date_created: 2018-12-11T12:00:05Z
date_published: 2012-07-01T00:00:00Z
date_updated: 2021-01-12T07:00:25Z
day: '01'
doi: 10.1016/j.phytochem.2012.03.012
extern: 1
intvolume: ' 79'
month: '07'
page: 67 - 77
publication: Phytochemistry
publication_status: published
publisher: Elsevier
publist_id: '3886'
quality_controlled: 0
status: public
title: Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants
that zeatin-O-glucoside is stored in the vacuole
type: journal_article
volume: 79
year: '2012'
...