--- _id: '2876' abstract: - lang: eng text: Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize β-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level. author: - first_name: Nagavalli full_name: Kiran, Nagavalli S last_name: Kiran - first_name: Eva full_name: Eva Benková id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Alena full_name: Reková, Alena last_name: Reková - first_name: Jaroslava full_name: Dubová, Jaroslava last_name: Dubová - first_name: Jiří full_name: Malbeck, Jiří last_name: Malbeck - first_name: Klaus full_name: Palme, Klaus last_name: Palme - first_name: Břetislav full_name: Brzobohatý, Břetislav last_name: Brzobohatý citation: ama: Kiran N, Benková E, Reková A, et al. Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole. Phytochemistry. 2012;79:67-77. doi:10.1016/j.phytochem.2012.03.012 apa: Kiran, N., Benková, E., Reková, A., Dubová, J., Malbeck, J., Palme, K., & Brzobohatý, B. (2012). Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole. Phytochemistry. Elsevier. https://doi.org/10.1016/j.phytochem.2012.03.012 chicago: Kiran, Nagavalli, Eva Benková, Alena Reková, Jaroslava Dubová, Jiří Malbeck, Klaus Palme, and Břetislav Brzobohatý. “Retargeting a Maize β-Glucosidase to the Vacuole - Evidence from Intact Plants That Zeatin-O-Glucoside Is Stored in the Vacuole.” Phytochemistry. Elsevier, 2012. https://doi.org/10.1016/j.phytochem.2012.03.012. ieee: N. Kiran et al., “Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole,” Phytochemistry, vol. 79. Elsevier, pp. 67–77, 2012. ista: Kiran N, Benková E, Reková A, Dubová J, Malbeck J, Palme K, Brzobohatý B. 2012. Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole. Phytochemistry. 79, 67–77. mla: Kiran, Nagavalli, et al. “Retargeting a Maize β-Glucosidase to the Vacuole - Evidence from Intact Plants That Zeatin-O-Glucoside Is Stored in the Vacuole.” Phytochemistry, vol. 79, Elsevier, 2012, pp. 67–77, doi:10.1016/j.phytochem.2012.03.012. short: N. Kiran, E. Benková, A. Reková, J. Dubová, J. Malbeck, K. Palme, B. Brzobohatý, Phytochemistry 79 (2012) 67–77. date_created: 2018-12-11T12:00:05Z date_published: 2012-07-01T00:00:00Z date_updated: 2021-01-12T07:00:25Z day: '01' doi: 10.1016/j.phytochem.2012.03.012 extern: 1 intvolume: ' 79' month: '07' page: 67 - 77 publication: Phytochemistry publication_status: published publisher: Elsevier publist_id: '3886' quality_controlled: 0 status: public title: Retargeting a maize β-glucosidase to the vacuole - Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole type: journal_article volume: 79 year: '2012' ...