---
_id: '288'
abstract:
- lang: eng
text: Recent lineage tracing studies have revealed that mammary gland homeostasis
relies on unipotent stem cells. However, whether and when lineage restriction
occurs during embryonic mammary development, and which signals orchestrate cell
fate specification, remain unknown. Using a combination of in vivo clonal analysis
with whole mount immunofluorescence and mathematical modelling of clonal dynamics,
we found that embryonic multipotent mammary cells become lineage-restricted surprisingly
early in development, with evidence for unipotency as early as E12.5 and no statistically
discernable bipotency after E15.5. To gain insights into the mechanisms governing
the switch from multipotency to unipotency, we used gain-of-function Notch1 mice
and demonstrated that Notch activation cell autonomously dictates luminal cell
fate specification to both embryonic and basally committed mammary cells. These
functional studies have important implications for understanding the signals underlying
cell plasticity and serve to clarify how reactivation of embryonic programs in
adult cells can lead to cancer.
article_processing_charge: No
article_type: original
author:
- first_name: Anna
full_name: Lilja, Anna
last_name: Lilja
- first_name: Veronica
full_name: Rodilla, Veronica
last_name: Rodilla
- first_name: Mathilde
full_name: Huyghe, Mathilde
last_name: Huyghe
- first_name: Edouard B
full_name: Hannezo, Edouard B
id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
last_name: Hannezo
orcid: 0000-0001-6005-1561
- first_name: Camille
full_name: Landragin, Camille
last_name: Landragin
- first_name: Olivier
full_name: Renaud, Olivier
last_name: Renaud
- first_name: Olivier
full_name: Leroy, Olivier
last_name: Leroy
- first_name: Steffen
full_name: Rulands, Steffen
last_name: Rulands
- first_name: Benjamin
full_name: Simons, Benjamin
last_name: Simons
- first_name: Silvia
full_name: Fré, Silvia
last_name: Fré
citation:
ama: Lilja A, Rodilla V, Huyghe M, et al. Clonal analysis of Notch1-expressing cells
reveals the existence of unipotent stem cells that retain long-term plasticity
in the embryonic mammary gland. Nature Cell Biology. 2018;20(6):677-687.
doi:10.1038/s41556-018-0108-1
apa: Lilja, A., Rodilla, V., Huyghe, M., Hannezo, E. B., Landragin, C., Renaud,
O., … Fré, S. (2018). Clonal analysis of Notch1-expressing cells reveals the existence
of unipotent stem cells that retain long-term plasticity in the embryonic mammary
gland. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/s41556-018-0108-1
chicago: Lilja, Anna, Veronica Rodilla, Mathilde Huyghe, Edouard B Hannezo, Camille
Landragin, Olivier Renaud, Olivier Leroy, Steffen Rulands, Benjamin Simons, and
Silvia Fré. “Clonal Analysis of Notch1-Expressing Cells Reveals the Existence
of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic Mammary
Gland.” Nature Cell Biology. Nature Publishing Group, 2018. https://doi.org/10.1038/s41556-018-0108-1.
ieee: A. Lilja et al., “Clonal analysis of Notch1-expressing cells reveals
the existence of unipotent stem cells that retain long-term plasticity in the
embryonic mammary gland,” Nature Cell Biology, vol. 20, no. 6. Nature Publishing
Group, pp. 677–687, 2018.
ista: Lilja A, Rodilla V, Huyghe M, Hannezo EB, Landragin C, Renaud O, Leroy O,
Rulands S, Simons B, Fré S. 2018. Clonal analysis of Notch1-expressing cells reveals
the existence of unipotent stem cells that retain long-term plasticity in the
embryonic mammary gland. Nature Cell Biology. 20(6), 677–687.
mla: Lilja, Anna, et al. “Clonal Analysis of Notch1-Expressing Cells Reveals the
Existence of Unipotent Stem Cells That Retain Long-Term Plasticity in the Embryonic
Mammary Gland.” Nature Cell Biology, vol. 20, no. 6, Nature Publishing
Group, 2018, pp. 677–87, doi:10.1038/s41556-018-0108-1.
short: A. Lilja, V. Rodilla, M. Huyghe, E.B. Hannezo, C. Landragin, O. Renaud, O.
Leroy, S. Rulands, B. Simons, S. Fré, Nature Cell Biology 20 (2018) 677–687.
date_created: 2018-12-11T11:45:38Z
date_published: 2018-05-21T00:00:00Z
date_updated: 2023-09-11T12:44:08Z
day: '21'
department:
- _id: EdHa
doi: 10.1038/s41556-018-0108-1
external_id:
isi:
- '000433237300003'
pmid:
- '29784917'
intvolume: ' 20'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984964
month: '05'
oa: 1
oa_version: Submitted Version
page: 677 - 687
pmid: 1
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '7594'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Clonal analysis of Notch1-expressing cells reveals the existence of unipotent
stem cells that retain long-term plasticity in the embryonic mammary gland
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 20
year: '2018'
...