--- res: bibo_abstract: - Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Bangjun foaf_name: Wang, Bangjun foaf_surname: Wang - foaf_Person: foaf_givenName: Aurélien foaf_name: Bailly, Aurélien foaf_surname: Bailly - foaf_Person: foaf_givenName: Marta foaf_name: Zwiewk, Marta foaf_surname: Zwiewk - foaf_Person: foaf_givenName: Sina foaf_name: Henrichs, Sina foaf_surname: Henrichs - foaf_Person: foaf_givenName: Elisa foaf_name: Azzarello, Elisa foaf_surname: Azzarello - foaf_Person: foaf_givenName: Stefano foaf_name: Mancuso, Stefano foaf_surname: Mancuso - foaf_Person: foaf_givenName: Masayoshi foaf_name: Maeshima, Masayoshi foaf_surname: Maeshima - foaf_Person: foaf_givenName: Jirí foaf_name: Friml, Jirí foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 - foaf_Person: foaf_givenName: Alexander foaf_name: Schulz, Alexander foaf_surname: Schulz - foaf_Person: foaf_givenName: Markus foaf_name: Geisler, Markus foaf_surname: Geisler bibo_doi: 10.1105/tpc.112.105999 bibo_issue: '1' bibo_volume: 25 dct_date: 2013^xs_gYear dct_language: eng dct_publisher: American Society of Plant Biologists@ dct_title: Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane@ fabio_hasPubmedId: '23321285' ...