---
_id: '2946'
abstract:
- lang: eng
  text: MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all
    cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them
    to specific target sites located in the 3′-untranslated region (3′-UTR) of target
    mRNAs leading to translational repression and deadenylation-induced mRNA degradation.
    Most miRNAs are processed from hairpin-structured precursors by the consecutive
    action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451
    is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we
    have characterized miR-451 sequence and structure requirements for processing
    as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears
    to be optimized for Ago2 cleavage and changes result in reduced processing. In
    addition, we show that the mature miR-451 only associates with Ago2 suggesting
    that mature miRNAs are not exchanged between different members of the Ago protein
    family. Based on cloning and deep sequencing of endogenous miRNAs associated with
    Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago
    identity appears to influence the length of some miRNAs, while others remain unaffected.
acknowledgement: "Deutsche Forschungsgemeinschaft (DFG) (SFB 960 and FOR855); European
  Research Council (ERC grant ‘sRNAs’); European Union (FP7 project ‘ONCOMIRs’); German
  Bundesministerium für Bildung und Forschung (BMBF, NGFN+, FKZ PIM-01GS0804-5); Bavarian
  Genome Research Network (BayGene to G.M.); The Netherlands Organization for Scientific
  Research (NWO, VIDI grant to E.B.). Funding for open access charge: DFG via the
  open access publishing program. \r\n\r\nWe thank Sigrun Ammon and Corinna Friederich
  for technical assistance and Sebastian Petri and Daniel Schraivogel for helpful
  discussions."
article_processing_charge: No
author:
- first_name: Anne
  full_name: Dueck, Anne
  last_name: Dueck
- first_name: Christian
  full_name: Ziegler, Christian
  last_name: Ziegler
- first_name: Alexander
  full_name: Eichner, Alexander
  id: 4DFA52AE-F248-11E8-B48F-1D18A9856A87
  last_name: Eichner
- first_name: Eugène
  full_name: Berezikov, Eugène
  last_name: Berezikov
- first_name: Gunter
  full_name: Meister, Gunter
  last_name: Meister
citation:
  ama: Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. MicroRNAs associated
    with the different human Argonaute proteins. <i>Nucleic Acids Research</i>. 2012;40(19):9850-9862.
    doi:<a href="https://doi.org/10.1093/nar/gks705">10.1093/nar/gks705</a>
  apa: Dueck, A., Ziegler, C., Eichner, A., Berezikov, E., &#38; Meister, G. (2012).
    MicroRNAs associated with the different human Argonaute proteins. <i>Nucleic Acids
    Research</i>. Oxford University Press. <a href="https://doi.org/10.1093/nar/gks705">https://doi.org/10.1093/nar/gks705</a>
  chicago: Dueck, Anne, Christian Ziegler, Alexander Eichner, Eugène Berezikov, and
    Gunter Meister. “MicroRNAs Associated with the Different Human Argonaute Proteins.”
    <i>Nucleic Acids Research</i>. Oxford University Press, 2012. <a href="https://doi.org/10.1093/nar/gks705">https://doi.org/10.1093/nar/gks705</a>.
  ieee: A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, and G. Meister, “MicroRNAs
    associated with the different human Argonaute proteins,” <i>Nucleic Acids Research</i>,
    vol. 40, no. 19. Oxford University Press, pp. 9850–9862, 2012.
  ista: Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. 2012. MicroRNAs associated
    with the different human Argonaute proteins. Nucleic Acids Research. 40(19), 9850–9862.
  mla: Dueck, Anne, et al. “MicroRNAs Associated with the Different Human Argonaute
    Proteins.” <i>Nucleic Acids Research</i>, vol. 40, no. 19, Oxford University Press,
    2012, pp. 9850–62, doi:<a href="https://doi.org/10.1093/nar/gks705">10.1093/nar/gks705</a>.
  short: A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, G. Meister, Nucleic Acids
    Research 40 (2012) 9850–9862.
corr_author: '1'
date_created: 2018-12-11T12:00:29Z
date_published: 2012-10-01T00:00:00Z
date_updated: 2025-09-30T08:12:07Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1093/nar/gks705
external_id:
  isi:
  - '000310377200046'
file:
- access_level: open_access
  checksum: 1bb8d1ff894014b481657a21083c941c
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:12Z
  date_updated: 2020-07-14T12:45:55Z
  file_id: '4993'
  file_name: IST-2015-383-v1+1_Nucl._Acids_Res.-2012-Dueck-9850-62.pdf
  file_size: 8126936
  relation: main_file
file_date_updated: 2020-07-14T12:45:55Z
has_accepted_license: '1'
intvolume: '        40'
isi: 1
issue: '19'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 9850 - 9862
publication: Nucleic Acids Research
publication_status: published
publisher: Oxford University Press
publist_id: '3786'
pubrep_id: '383'
quality_controlled: '1'
scopus_import: '1'
status: public
title: MicroRNAs associated with the different human Argonaute proteins
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 40
year: '2012'
...