article published yes Alejandro Pernia-Andrade author 36963E98-F248-11E8-B48F-1D18A9856A87 Sarit Goswami author 3A578F32-F248-11E8-B48F-1D18A9856A87 Yvonne Stickler author 63B76600-E9CC-11E9-9B5F-82450873F7A1 Ulrich Fröbe author Alois Schlögl author 45BF87EE-F248-11E8-B48F-1D18A9856A870000-0002-5621-8100 Peter M Jonas author 353C1B58-F248-11E8-B48F-1D18A9856A870000-0001-5001-4804 PeJo department ScienComp department Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen project Spontaneous postsynaptic currents (PSCs) provide key information about the mechanisms of synaptic transmission and the activity modes of neuronal networks. However, detecting spontaneous PSCs in vitro and in vivo has been challenging, because of the small amplitude, the variable kinetics, and the undefined time of generation of these events. Here, we describe a, to our knowledge, new method for detecting spontaneous synaptic events by deconvolution, using a template that approximates the average time course of spontaneous PSCs. A recorded PSC trace is deconvolved from the template, resulting in a series of delta-like functions. The maxima of these delta-like events are reliably detected, revealing the precise onset times of the spontaneous PSCs. Among all detection methods, the deconvolution-based method has a unique temporal resolution, allowing the detection of individual events in high-frequency bursts. Furthermore, the deconvolution-based method has a high amplitude resolution, because deconvolution can substantially increase the signal/noise ratio. When tested against previously published methods using experimental data, the deconvolution-based method was superior for spontaneous PSCs recorded in vivo. Using the high-resolution deconvolution-based detection algorithm, we show that the frequency of spontaneous excitatory postsynaptic currents in dentate gyrus granule cells is 4.5 times higher in vivo than in vitro. Biophysical Society2012 eng 23062335 00030957140000510.1016/j.bpj.2012.08.039 10371429 - 1439 A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, and P. M. Jonas, “A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo,” <i>Biophysical Journal</i>, vol. 103, no. 7. Biophysical Society, pp. 1429–1439, 2012. Pernia-Andrade, A., Goswami, S., Stickler, Y., Fröbe, U., Schlögl, A., &#38; Jonas, P. M. (2012). A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. <i>Biophysical Journal</i>. Biophysical Society. <a href="https://doi.org/10.1016/j.bpj.2012.08.039">https://doi.org/10.1016/j.bpj.2012.08.039</a> A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, P.M. Jonas, Biophysical Journal 103 (2012) 1429–1439. Pernia-Andrade, Alejandro, Sarit Goswami, Yvonne Stickler, Ulrich Fröbe, Alois Schlögl, and Peter M Jonas. “A Deconvolution Based Method with High Sensitivity and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro and in Vivo.” <i>Biophysical Journal</i>. Biophysical Society, 2012. <a href="https://doi.org/10.1016/j.bpj.2012.08.039">https://doi.org/10.1016/j.bpj.2012.08.039</a>. Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. <i>Biophysical Journal</i>. 2012;103(7):1429-1439. doi:<a href="https://doi.org/10.1016/j.bpj.2012.08.039">10.1016/j.bpj.2012.08.039</a> Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. 2012. A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal. 103(7), 1429–1439. Pernia-Andrade, Alejandro, et al. “A Deconvolution Based Method with High Sensitivity and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro and in Vivo.” <i>Biophysical Journal</i>, vol. 103, no. 7, Biophysical Society, 2012, pp. 1429–39, doi:<a href="https://doi.org/10.1016/j.bpj.2012.08.039">10.1016/j.bpj.2012.08.039</a>. 29542018-12-11T12:00:32Z2026-07-06T12:32:51Z