---
res:
  bibo_abstract:
  - Plant biology is currently experiencing a growing demand for easy and reliable
    mRNA and protein localisation techniques. Here, we present novel whole mount in
    situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs
    and proteins in Arabidopsis seedlings. We demonstrate that these methods can be
    used in different organs of Arabidopsis seedlings as well as in other plant species.
    In order to achieve better reproducibility and higher throughput, we modified
    these protocols for automation to be performed by a liquid handling robot. In
    addition, we show that other procedures such as reporter enzyme assays and tissue
    clearing can be similarly automated. We present examples of application of our
    protocols including mRNA localisation and proteins and epitope tag (co)localisations
    which demonstrate that these methods provide reliable and versatile tools for
    expression, localisation and anatomical studies in plants.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jirí
      foaf_name: Friml, Jirí
      foaf_surname: Friml
      foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8302-7596
  - foaf_Person:
      foaf_givenName: Eva
      foaf_name: Benková, Eva
      foaf_surname: Benková
      foaf_workInfoHomepage: http://www.librecat.org/personId=38F4F166-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0002-8510-9739
  - foaf_Person:
      foaf_givenName: Ulrike
      foaf_name: Mayer, Ulrike
      foaf_surname: Mayer
  - foaf_Person:
      foaf_givenName: Klaus
      foaf_name: Palme, Klaus
      foaf_surname: Palme
  - foaf_Person:
      foaf_givenName: Gerhard
      foaf_name: Muster, Gerhard
      foaf_surname: Muster
  bibo_doi: 10.1046/j.1365-313X.2003.01705.x
  bibo_issue: '1'
  bibo_volume: 34
  dct_date: 2003^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0960-7412
  - http://id.crossref.org/issn/1365-313X
  dct_language: eng
  dct_publisher: Wiley@
  dct_title: Automated whole mount localisation techniques for plant seedlings@
  fabio_hasPubmedId: '12662314'
...