---
OA_type: closed access
_id: '2993'
abstract:
- lang: eng
  text: Plant biology is currently experiencing a growing demand for easy and reliable
    mRNA and protein localisation techniques. Here, we present novel whole mount in
    situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs
    and proteins in Arabidopsis seedlings. We demonstrate that these methods can be
    used in different organs of Arabidopsis seedlings as well as in other plant species.
    In order to achieve better reproducibility and higher throughput, we modified
    these protocols for automation to be performed by a liquid handling robot. In
    addition, we show that other procedures such as reporter enzyme assays and tissue
    clearing can be similarly automated. We present examples of application of our
    protocols including mRNA localisation and proteins and epitope tag (co)localisations
    which demonstrate that these methods provide reliable and versatile tools for
    expression, localisation and anatomical studies in plants.
article_processing_charge: No
article_type: original
author:
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Ulrike
  full_name: Mayer, Ulrike
  last_name: Mayer
- first_name: Klaus
  full_name: Palme, Klaus
  last_name: Palme
- first_name: Gerhard
  full_name: Muster, Gerhard
  last_name: Muster
citation:
  ama: Friml J, Benková E, Mayer U, Palme K, Muster G. Automated whole mount localisation
    techniques for plant seedlings. <i>Plant Journal</i>. 2003;34(1):115-124. doi:<a
    href="https://doi.org/10.1046/j.1365-313X.2003.01705.x">10.1046/j.1365-313X.2003.01705.x</a>
  apa: Friml, J., Benková, E., Mayer, U., Palme, K., &#38; Muster, G. (2003). Automated
    whole mount localisation techniques for plant seedlings. <i>Plant Journal</i>.
    Wiley. <a href="https://doi.org/10.1046/j.1365-313X.2003.01705.x">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>
  chicago: Friml, Jiří, Eva Benková, Ulrike Mayer, Klaus Palme, and Gerhard Muster.
    “Automated Whole Mount Localisation Techniques for Plant Seedlings.” <i>Plant
    Journal</i>. Wiley, 2003. <a href="https://doi.org/10.1046/j.1365-313X.2003.01705.x">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>.
  ieee: J. Friml, E. Benková, U. Mayer, K. Palme, and G. Muster, “Automated whole
    mount localisation techniques for plant seedlings,” <i>Plant Journal</i>, vol.
    34, no. 1. Wiley, pp. 115–124, 2003.
  ista: Friml J, Benková E, Mayer U, Palme K, Muster G. 2003. Automated whole mount
    localisation techniques for plant seedlings. Plant Journal. 34(1), 115–124.
  mla: Friml, Jiří, et al. “Automated Whole Mount Localisation Techniques for Plant
    Seedlings.” <i>Plant Journal</i>, vol. 34, no. 1, Wiley, 2003, pp. 115–24, doi:<a
    href="https://doi.org/10.1046/j.1365-313X.2003.01705.x">10.1046/j.1365-313X.2003.01705.x</a>.
  short: J. Friml, E. Benková, U. Mayer, K. Palme, G. Muster, Plant Journal 34 (2003)
    115–124.
date_created: 2018-12-11T12:00:44Z
date_published: 2003-04-01T00:00:00Z
date_updated: 2026-05-22T09:01:28Z
day: '01'
doi: 10.1046/j.1365-313X.2003.01705.x
extern: '1'
external_id:
  pmid:
  - '12662314'
fulldoi: https://doi.org/10.1046/j.1365-313X.2003.01705.x
intvolume: '        34'
issue: '1'
language:
- iso: eng
month: '04'
oa_version: None
page: 115 - 124
pmid: 1
publication: Plant Journal
publication_identifier:
  eissn:
  - 1365-313X
  issn:
  - 0960-7412
publication_status: published
publisher: Wiley
publist_id: '3709'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Automated whole mount localisation techniques for plant seedlings
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 34
year: '2003'
...