{"quality_controlled":0,"date_published":"2005-06-30T00:00:00Z","author":[{"first_name":"Tomasz","last_name":"Paciorek","full_name":"Paciorek, Tomasz"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"},{"first_name":"Nadia","last_name":"Ruthardt","full_name":"Ruthardt, Nadia"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"first_name":"York","last_name":"Stierhof","full_name":"Stierhof, York-Dieter"},{"first_name":"Jürgen","last_name":"Kleine Vehn","full_name":"Kleine-Vehn, Jürgen"},{"last_name":"Morris","first_name":"David","full_name":"Morris, David A"},{"first_name":"Neil","last_name":"Emans","full_name":"Emans, Neil"},{"full_name":"Jürgens, Gerd","first_name":"Gerd","last_name":"Jürgens"},{"first_name":"Niko","last_name":"Geldner","full_name":"Geldner, Niko"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Jirí Friml","last_name":"Friml","first_name":"Jirí"}],"publication":"Nature","publication_status":"published","doi":"10.1038/nature03633","month":"06","_id":"3001","date_updated":"2021-01-12T07:40:23Z","date_created":"2018-12-11T12:00:47Z","publist_id":"3702","intvolume":"       435","extern":1,"issue":"7046","year":"2005","page":"1251 - 1256","title":"Auxin inhibits endocytosis and promotes its own efflux from cells","type":"journal_article","day":"30","citation":{"apa":"Paciorek, T., Zažímalová, E., Ruthardt, N., Petrášek, J., Stierhof, Y., Kleine Vehn, J., … Friml, J. (2005). Auxin inhibits endocytosis and promotes its own efflux from cells. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature03633\">https://doi.org/10.1038/nature03633</a>","ama":"Paciorek T, Zažímalová E, Ruthardt N, et al. Auxin inhibits endocytosis and promotes its own efflux from cells. <i>Nature</i>. 2005;435(7046):1251-1256. doi:<a href=\"https://doi.org/10.1038/nature03633\">10.1038/nature03633</a>","short":"T. Paciorek, E. Zažímalová, N. Ruthardt, J. Petrášek, Y. Stierhof, J. Kleine Vehn, D. Morris, N. Emans, G. Jürgens, N. Geldner, J. Friml, Nature 435 (2005) 1251–1256.","mla":"Paciorek, Tomasz, et al. “Auxin Inhibits Endocytosis and Promotes Its Own Efflux from Cells.” <i>Nature</i>, vol. 435, no. 7046, Nature Publishing Group, 2005, pp. 1251–56, doi:<a href=\"https://doi.org/10.1038/nature03633\">10.1038/nature03633</a>.","ista":"Paciorek T, Zažímalová E, Ruthardt N, Petrášek J, Stierhof Y, Kleine Vehn J, Morris D, Emans N, Jürgens G, Geldner N, Friml J. 2005. Auxin inhibits endocytosis and promotes its own efflux from cells. Nature. 435(7046), 1251–1256.","chicago":"Paciorek, Tomasz, Eva Zažímalová, Nadia Ruthardt, Jan Petrášek, York Stierhof, Jürgen Kleine Vehn, David Morris, et al. “Auxin Inhibits Endocytosis and Promotes Its Own Efflux from Cells.” <i>Nature</i>. Nature Publishing Group, 2005. <a href=\"https://doi.org/10.1038/nature03633\">https://doi.org/10.1038/nature03633</a>.","ieee":"T. Paciorek <i>et al.</i>, “Auxin inhibits endocytosis and promotes its own efflux from cells,” <i>Nature</i>, vol. 435, no. 7046. Nature Publishing Group, pp. 1251–1256, 2005."},"volume":435,"status":"public","publisher":"Nature Publishing Group","abstract":[{"text":"One of the mechanisms by which signalling molecules regulate cellular behaviour is modulating subcellular protein translocation. This mode of regulation is often based on specialized vesicle trafficking, termed constitutive cycling, which consists of repeated internalization and recycling of proteins to and from the plasma membrane. No such mechanism of hormone action has been shown in plants although several proteins, including the PIN auxin efflux facilitators, exhibit constitutive cycling. Here we show that a major regulator of plant development, auxin, inhibits endocytosis. This effect is specific to biologically active auxins and requires activity of the Calossin-like protein BIG. By inhibiting the internalization step of PIN constitutive cycling, auxin increases levels of PINs at the plasma membrane. Concomitantly, auxin promotes its own efflux from cells by a vesicle-trafficking-dependent mechanism. Furthermore, asymmetric auxin translocation during gravitropism is correlated with decreased PIN internalization. Our data imply a previously undescribed mode of plant hormone action: by modulating PIN protein trafficking, auxin regulates PIN abundance and activity at the cell surface, providing a mechanism for the feedback regulation of auxin transport.","lang":"eng"}]}