{"volume":312,"status":"public","_id":"3012","publication":"Science","publisher":"American Association for the Advancement of Science","quality_controlled":0,"date_updated":"2021-01-12T07:40:27Z","type":"journal_article","publication_status":"published","day":"12","year":"2006","issue":"5775","page":"914 - 918","intvolume":"       312","title":"PIN proteins perform a rate-limiting function in cellular auxin efflux","extern":1,"month":"05","author":[{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"first_name":"Jozef","last_name":"Mravec","full_name":"Mravec, Jozef"},{"full_name":"Bouchard, Rodolphe","last_name":"Bouchard","first_name":"Rodolphe"},{"last_name":"Blakeslee","full_name":"Blakeslee, Joshua","first_name":"Joshua"},{"first_name":"Melinda F","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","full_name":"Melinda Abas","last_name":"Abas"},{"last_name":"Seifertová","full_name":"Seifertová, Daniela","first_name":"Daniela"},{"full_name":"Wiśniewska, Justyna","last_name":"Wiśniewska","first_name":"Justyna"},{"full_name":"Tadele, Zerihun","last_name":"Tadele","first_name":"Zerihun"},{"first_name":"Martin","full_name":"Kubeš, Martin","last_name":"Kubeš"},{"first_name":"Milada","last_name":"Čovanová","full_name":"Čovanová, Milada"},{"first_name":"Pankaj","last_name":"Dhonukshe","full_name":"Dhonukshe, Pankaj"},{"full_name":"Skůpa, Petr","last_name":"Skůpa","first_name":"Petr"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Eva Benková"},{"full_name":"Perry, Lucie","last_name":"Perry","first_name":"Lucie"},{"first_name":"Pavel","last_name":"Křeček","full_name":"Křeček, Pavel"},{"first_name":"Ok","last_name":"Lee","full_name":"Lee, Ok Ran"},{"first_name":"Gerald","last_name":"Fink","full_name":"Fink, Gerald R"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"},{"full_name":"Murphy, Angus S","last_name":"Murphy","first_name":"Angus"},{"first_name":"Christian","full_name":"Luschnig, Christian","last_name":"Luschnig"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Jirí Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"doi":"10.1126/science.1123542","date_created":"2018-12-11T12:00:51Z","publist_id":"3690","citation":{"ista":"Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 312(5775), 914–918.","ieee":"J. Petrášek <i>et al.</i>, “PIN proteins perform a rate-limiting function in cellular auxin efflux,” <i>Science</i>, vol. 312, no. 5775. American Association for the Advancement of Science, pp. 914–918, 2006.","chicago":"Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” <i>Science</i>. American Association for the Advancement of Science, 2006. <a href=\"https://doi.org/10.1126/science.1123542\">https://doi.org/10.1126/science.1123542</a>.","mla":"Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” <i>Science</i>, vol. 312, no. 5775, American Association for the Advancement of Science, 2006, pp. 914–18, doi:<a href=\"https://doi.org/10.1126/science.1123542\">10.1126/science.1123542</a>.","short":"J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová, J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková, L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová, J. Friml, Science 312 (2006) 914–918.","ama":"Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. <i>Science</i>. 2006;312(5775):914-918. doi:<a href=\"https://doi.org/10.1126/science.1123542\">10.1126/science.1123542</a>","apa":"Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová, D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1123542\">https://doi.org/10.1126/science.1123542</a>"},"abstract":[{"lang":"eng","text":"Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux."}],"date_published":"2006-05-12T00:00:00Z"}