--- _id: '3012' abstract: - lang: eng text: Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux. author: - first_name: Jan full_name: Petrášek, Jan last_name: Petrášek - first_name: Jozef full_name: Mravec, Jozef last_name: Mravec - first_name: Rodolphe full_name: Bouchard, Rodolphe last_name: Bouchard - first_name: Joshua full_name: Blakeslee, Joshua last_name: Blakeslee - first_name: Melinda F full_name: Melinda Abas id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87 last_name: Abas - first_name: Daniela full_name: Seifertová, Daniela last_name: Seifertová - first_name: Justyna full_name: Wiśniewska, Justyna last_name: Wiśniewska - first_name: Zerihun full_name: Tadele, Zerihun last_name: Tadele - first_name: Martin full_name: Kubeš, Martin last_name: Kubeš - first_name: Milada full_name: Čovanová, Milada last_name: Čovanová - first_name: Pankaj full_name: Dhonukshe, Pankaj last_name: Dhonukshe - first_name: Petr full_name: Skůpa, Petr last_name: Skůpa - first_name: Eva full_name: Eva Benková id: 38F4F166-F248-11E8-B48F-1D18A9856A87 last_name: Benková orcid: 0000-0002-8510-9739 - first_name: Lucie full_name: Perry, Lucie last_name: Perry - first_name: Pavel full_name: Křeček, Pavel last_name: Křeček - first_name: Ok full_name: Lee, Ok Ran last_name: Lee - first_name: Gerald full_name: Fink, Gerald R last_name: Fink - first_name: Markus full_name: Geisler, Markus last_name: Geisler - first_name: Angus full_name: Murphy, Angus S last_name: Murphy - first_name: Christian full_name: Luschnig, Christian last_name: Luschnig - first_name: Eva full_name: Zažímalová, Eva last_name: Zažímalová - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542 apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová, D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1123542 chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542. ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association for the Advancement of Science, pp. 914–918, 2006. ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 312(5775), 914–918. mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542. short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová, J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková, L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová, J. Friml, Science 312 (2006) 914–918. date_created: 2018-12-11T12:00:51Z date_published: 2006-05-12T00:00:00Z date_updated: 2021-01-12T07:40:27Z day: '12' doi: 10.1126/science.1123542 extern: 1 intvolume: ' 312' issue: '5775' month: '05' page: 914 - 918 publication: Science publication_status: published publisher: American Association for the Advancement of Science publist_id: '3690' quality_controlled: 0 status: public title: PIN proteins perform a rate-limiting function in cellular auxin efflux type: journal_article volume: 312 year: '2006' ...