---
_id: '3012'
abstract:
- lang: eng
text: Intercellular flow of the phytohormone auxin underpins multiple developmental
processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein
(PGP) transporters are crucial factors in auxin transport-related development,
yet the molecular function of PINs remains unknown. Here, we show that PINs mediate
auxin efflux from mammalian and yeast cells without needing additional plant-specific
factors. Conditional gain-of-function alleles and quantitative measurements of
auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the
action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to
auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement
of PINs in catalyzing cellular auxin efflux.
author:
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Rodolphe
full_name: Bouchard, Rodolphe
last_name: Bouchard
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Melinda F
full_name: Melinda Abas
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Zerihun
full_name: Tadele, Zerihun
last_name: Tadele
- first_name: Martin
full_name: Kubeš, Martin
last_name: Kubeš
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Lucie
full_name: Perry, Lucie
last_name: Perry
- first_name: Pavel
full_name: Křeček, Pavel
last_name: Křeček
- first_name: Ok
full_name: Lee, Ok Ran
last_name: Lee
- first_name: Gerald
full_name: Fink, Gerald R
last_name: Fink
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting
function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542
apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová,
D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular
auxin efflux. Science. American Association for the Advancement of Science.
https://doi.org/10.1126/science.1123542
chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda
F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a
Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association
for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542.
ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function
in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association
for the Advancement of Science, pp. 914–918, 2006.
ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska
J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček
P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006.
PIN proteins perform a rate-limiting function in cellular auxin efflux. Science.
312(5775), 914–918.
mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular
Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the
Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542.
short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová,
J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková,
L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová,
J. Friml, Science 312 (2006) 914–918.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1123542
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
page: 914 - 918
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3690'
quality_controlled: 0
status: public
title: PIN proteins perform a rate-limiting function in cellular auxin efflux
type: journal_article
volume: 312
year: '2006'
...