--- res: bibo_abstract: - 'There is a growing demand for methods that allow rapid and reliable in situ localization of proteins in plant cells. The immunocytochemistry protocol presented here can be used routinely to observe protein localization patterns in tissue sections of various plant species. This protocol is especially suitable for plant species with more-complex tissue architecture (such as maize, Zea mays), which makes it difficult to use an easier whole-mount procedure for protein localization. To facilitate the antibody-antigen reaction, it is necessary to include a wax-embedding and tissue-sectioning step. The protocol consists of the following procedures: chemical fixation of tissue, dehydration, wax embedding, sectioning, dewaxing, rehydration, blocking and antibody incubation. The detailed protocol, recommended controls and troubleshooting are presented here, along with examples of applications.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Tomasz foaf_name: Paciorek, Tomasz foaf_surname: Paciorek - foaf_Person: foaf_givenName: Michael foaf_name: Sauer, Michael foaf_surname: Sauer - foaf_Person: foaf_givenName: Jozef foaf_name: Balla, Jozef foaf_surname: Balla - foaf_Person: foaf_givenName: Justyna foaf_name: Wiśniewska, Justyna foaf_surname: Wiśniewska - foaf_Person: foaf_givenName: Jirí foaf_name: Jirí Friml foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1038/nprot.2006.16 bibo_issue: '1' bibo_volume: 1 dct_date: 2006^xs_gYear dct_publisher: Nature Publishing Group@ dct_title: Immunocytochemical technique for protein localization in sections of plant tissues@ ...