--- res: bibo_abstract: - 'As the field of plant molecular biology is swiftly advancing, a need has been created for methods that allow rapid and reliable in situ localization of proteins in plant cells. Here we describe a whole-mount ''immunolocalization'' technique for various plant tissues, including roots, hypocotyls, cotyledons, young primary leaves and embryos of Arabidopsis thaliana and other species. The detailed protocol, recommended controls and troubleshooting are presented, along with examples of applications. The protocol consists of five main procedures: tissue fixation, tissue permeation, blocking, primary and secondary antibody incubation. Notably, the first procedure (tissue fixation) includes several steps (4-12) that are absolutely necessary for protein localization in hypocotyls, cotyledons and young primary leaves but should be omitted for other tissues. The protocol is usually done in 3 days, but could also be completed in 2 days.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Michael foaf_name: Sauer, Michael foaf_surname: Sauer - foaf_Person: foaf_givenName: Tomasz foaf_name: Paciorek, Tomasz foaf_surname: Paciorek - foaf_Person: foaf_givenName: Eva foaf_name: Eva Benková foaf_surname: Benková foaf_workInfoHomepage: http://www.librecat.org/personId=38F4F166-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8510-9739 - foaf_Person: foaf_givenName: Jirí foaf_name: Jirí Friml foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1038/nprot.2006.15 bibo_issue: '1' bibo_volume: 1 dct_date: 2006^xs_gYear dct_publisher: Nature Publishing Group@ dct_title: Immunocytochemical techniques for whole mount in situ protein localization in plants@ ...