--- _id: '3078' abstract: - lang: eng text: Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in many cases recombinant proteins with fluorescent proteins can fulfill this task, antibody-based immunological detection of proteins is a complementary technique, which avoids the risk of inducing side effects by a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity. Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants. The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species. This chapter describes a whole mount immunolocalization protocol, which has been extensively used in Arabidopsis roots and some above-ground tissues, and that also works in other species. Additionally, for bulky or hard tissue types, a variation of this protocol for paraffin-embedded sections is given. alternative_title: - Methods in Molecular Biology author: - first_name: Michael full_name: Sauer, Michael last_name: Sauer - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: 'Sauer M, Friml J. Immunolocalization of proteins in plants . In: Hennig L, Köhler C, eds. Plant Developmental Biology. Vol 655. Humana Press; 2010:253-263. doi:10.1007/978-1-60761-765-5_17' apa: Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants . In L. Hennig & C. Köhler (Eds.), Plant Developmental Biology (Vol. 655, pp. 253–263). Humana Press. https://doi.org/10.1007/978-1-60761-765-5_17 chicago: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .” In Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, 655:253–63. Humana Press, 2010. https://doi.org/10.1007/978-1-60761-765-5_17. ieee: M. Sauer and J. Friml, “Immunolocalization of proteins in plants ,” in Plant Developmental Biology, vol. 655, L. Hennig and C. Köhler, Eds. Humana Press, 2010, pp. 253–263. ista: 'Sauer M, Friml J. 2010.Immunolocalization of proteins in plants . In: Plant Developmental Biology. Methods in Molecular Biology, vol. 655, 253–263.' mla: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .” Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler, vol. 655, Humana Press, 2010, pp. 253–63, doi:10.1007/978-1-60761-765-5_17. short: M. Sauer, J. Friml, in:, L. Hennig, C. Köhler (Eds.), Plant Developmental Biology, Humana Press, 2010, pp. 253–263. date_created: 2018-12-11T12:01:14Z date_published: 2010-08-12T00:00:00Z date_updated: 2021-01-12T07:40:53Z day: '12' doi: 10.1007/978-1-60761-765-5_17 editor: - first_name: Lars full_name: Hennig, Lars last_name: Hennig - first_name: Claudia full_name: Köhler, Claudia last_name: Köhler extern: 1 intvolume: ' 655' month: '08' page: 253 - 263 publication: Plant Developmental Biology publication_status: published publisher: Humana Press publist_id: '3623' quality_controlled: 0 status: public title: 'Immunolocalization of proteins in plants ' type: book_chapter volume: 655 year: '2010' ...