---
_id: '3078'
abstract:
- lang: eng
text: Rapid advances in the field of plant biology, especially in plant cell biology,
have created the need for methods that allow the localization of proteins in situ
at subcellular resolution. Although in many cases recombinant proteins with fluorescent
proteins can fulfill this task, antibody-based immunological detection of proteins
is a complementary technique, which avoids the risk of inducing side effects by
a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity.
Moreover, recombinant protein techniques are applicable only to a rather limited
set of model plants. The immunolocalization protocols presented here can be used
to display protein localization patterns in different tissues of various plant
species. This chapter describes a whole mount immunolocalization protocol, which
has been extensively used in Arabidopsis roots and some above-ground tissues,
and that also works in other species. Additionally, for bulky or hard tissue types,
a variation of this protocol for paraffin-embedded sections is given.
alternative_title:
- Methods in Molecular Biology
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sauer M, Friml J. Immunolocalization of proteins in plants . In: Hennig L,
Köhler C, eds. Plant Developmental Biology. Vol 655. Humana Press; 2010:253-263.
doi:10.1007/978-1-60761-765-5_17'
apa: Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants
. In L. Hennig & C. Köhler (Eds.), Plant Developmental Biology (Vol.
655, pp. 253–263). Humana Press. https://doi.org/10.1007/978-1-60761-765-5_17
chicago: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants
.” In Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler,
655:253–63. Humana Press, 2010. https://doi.org/10.1007/978-1-60761-765-5_17.
ieee: M. Sauer and J. Friml, “Immunolocalization of proteins in plants ,” in Plant
Developmental Biology, vol. 655, L. Hennig and C. Köhler, Eds. Humana Press,
2010, pp. 253–263.
ista: 'Sauer M, Friml J. 2010.Immunolocalization of proteins in plants . In: Plant
Developmental Biology. Methods in Molecular Biology, vol. 655, 253–263.'
mla: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .”
Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler,
vol. 655, Humana Press, 2010, pp. 253–63, doi:10.1007/978-1-60761-765-5_17.
short: M. Sauer, J. Friml, in:, L. Hennig, C. Köhler (Eds.), Plant Developmental
Biology, Humana Press, 2010, pp. 253–263.
date_created: 2018-12-11T12:01:14Z
date_published: 2010-08-12T00:00:00Z
date_updated: 2021-01-12T07:40:53Z
day: '12'
doi: 10.1007/978-1-60761-765-5_17
editor:
- first_name: Lars
full_name: Hennig, Lars
last_name: Hennig
- first_name: Claudia
full_name: Köhler, Claudia
last_name: Köhler
extern: 1
intvolume: ' 655'
month: '08'
page: 253 - 263
publication: Plant Developmental Biology
publication_status: published
publisher: Humana Press
publist_id: '3623'
quality_controlled: 0
status: public
title: 'Immunolocalization of proteins in plants '
type: book_chapter
volume: 655
year: '2010'
...