--- _id: '3081' abstract: - lang: eng text: Endocytosis is crucial for various cellular functions and development of multicellular organisms. In mammals and yeast, ADP-ribosylation factor (ARF) GTPases, key components of vesicle formation, and their regulators ARF-guanine nucleotide exchange factors (GEFs) and ARF-GTPase-activating protein (GAPs) mediate endocytosis. A similar role has not been established in plants,mainly because of the lack of the canonical ARF and ARF-GEF components that are involved in endocytosis in other eukaryotes. In this study, we revealed a regulatory mechanism of endocytosis in plants based on ARF GTPase activity.Weidentified that ARF-GEFGNOMand ARF-GAP VASCULAR NETWORK DEFECTIVE 3 (VAN3), both of which are involved in polar auxin transport-dependent morphogenesis, localize at the plasma membranes as well as in intracellular structures. Variable angle epifluorescence microscopy revealed that GNOM and VAN3 localize to partially overlapping discrete foci at the plasmamembranes that are regularly associated with the endocytic vesicle coat clathrin. Genetic studies revealed that GNOM and VAN3 activities are required for endocytosis and internalization of plasma membrane proteins, including PIN-FORMED auxin transporters. These findings identified ARF GTPase-based regulatory mechanisms for endocytosis in plants. GNOMand VAN3 previously were proposed to function solely at the recycling endosomes and trans-Golgi networks, respectively. Therefore our findings uncovered an additional cellular function of these prominent developmental regulators. author: - first_name: Satoshi full_name: Naramoto, Satoshi last_name: Naramoto - first_name: Jürgen full_name: Kleine-Vehn, Jürgen last_name: Kleine Vehn - first_name: Stéphanie full_name: Robert, Stéphanie last_name: Robert - first_name: Masaru full_name: Fujimoto, Masaru last_name: Fujimoto - first_name: Tomoko full_name: Dainobu, Tomoko last_name: Dainobu - first_name: Tomasz full_name: Paciorek, Tomasz last_name: Paciorek - first_name: Takashi full_name: Ueda, Takashi last_name: Ueda - first_name: Akihiko full_name: Nakano, Akihiko last_name: Nakano - first_name: Marc full_name: Van Montagu, Marc C last_name: Van Montagu - first_name: Hiroo full_name: Fukuda, Hiroo last_name: Fukuda - first_name: Jirí full_name: Jirí Friml id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 citation: ama: Naramoto S, Kleine Vehn J, Robert S, et al. ADP ribosylation factor machinery mediates endocytosis in plant cells. PNAS. 2010;107(50):21890-21895. doi:10.1073/pnas.1016260107 apa: Naramoto, S., Kleine Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., … Friml, J. (2010). ADP ribosylation factor machinery mediates endocytosis in plant cells. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1016260107 chicago: Naramoto, Satoshi, Jürgen Kleine Vehn, Stéphanie Robert, Masaru Fujimoto, Tomoko Dainobu, Tomasz Paciorek, Takashi Ueda, et al. “ADP Ribosylation Factor Machinery Mediates Endocytosis in Plant Cells.” PNAS. National Academy of Sciences, 2010. https://doi.org/10.1073/pnas.1016260107. ieee: S. Naramoto et al., “ADP ribosylation factor machinery mediates endocytosis in plant cells,” PNAS, vol. 107, no. 50. National Academy of Sciences, pp. 21890–21895, 2010. ista: Naramoto S, Kleine Vehn J, Robert S, Fujimoto M, Dainobu T, Paciorek T, Ueda T, Nakano A, Van Montagu M, Fukuda H, Friml J. 2010. ADP ribosylation factor machinery mediates endocytosis in plant cells. PNAS. 107(50), 21890–21895. mla: Naramoto, Satoshi, et al. “ADP Ribosylation Factor Machinery Mediates Endocytosis in Plant Cells.” PNAS, vol. 107, no. 50, National Academy of Sciences, 2010, pp. 21890–95, doi:10.1073/pnas.1016260107. short: S. Naramoto, J. Kleine Vehn, S. Robert, M. Fujimoto, T. Dainobu, T. Paciorek, T. Ueda, A. Nakano, M. Van Montagu, H. Fukuda, J. Friml, PNAS 107 (2010) 21890–21895. date_created: 2018-12-11T12:01:15Z date_published: 2010-12-14T00:00:00Z date_updated: 2021-01-12T07:40:55Z day: '14' doi: 10.1073/pnas.1016260107 extern: 1 intvolume: ' 107' issue: '50' month: '12' page: 21890 - 21895 publication: PNAS publication_status: published publisher: National Academy of Sciences publist_id: '3621' quality_controlled: 0 status: public title: ADP ribosylation factor machinery mediates endocytosis in plant cells type: journal_article volume: 107 year: '2010' ...