---
_id: '3081'
abstract:
- lang: eng
text: Endocytosis is crucial for various cellular functions and development of multicellular
organisms. In mammals and yeast, ADP-ribosylation factor (ARF) GTPases, key components
of vesicle formation, and their regulators ARF-guanine nucleotide exchange factors
(GEFs) and ARF-GTPase-activating protein (GAPs) mediate endocytosis. A similar
role has not been established in plants,mainly because of the lack of the canonical
ARF and ARF-GEF components that are involved in endocytosis in other eukaryotes.
In this study, we revealed a regulatory mechanism of endocytosis in plants based
on ARF GTPase activity.Weidentified that ARF-GEFGNOMand ARF-GAP VASCULAR NETWORK
DEFECTIVE 3 (VAN3), both of which are involved in polar auxin transport-dependent
morphogenesis, localize at the plasma membranes as well as in intracellular structures.
Variable angle epifluorescence microscopy revealed that GNOM and VAN3 localize
to partially overlapping discrete foci at the plasmamembranes that are regularly
associated with the endocytic vesicle coat clathrin. Genetic studies revealed
that GNOM and VAN3 activities are required for endocytosis and internalization
of plasma membrane proteins, including PIN-FORMED auxin transporters. These findings
identified ARF GTPase-based regulatory mechanisms for endocytosis in plants. GNOMand
VAN3 previously were proposed to function solely at the recycling endosomes and
trans-Golgi networks, respectively. Therefore our findings uncovered an additional
cellular function of these prominent developmental regulators.
author:
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Masaru
full_name: Fujimoto, Masaru
last_name: Fujimoto
- first_name: Tomoko
full_name: Dainobu, Tomoko
last_name: Dainobu
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Takashi
full_name: Ueda, Takashi
last_name: Ueda
- first_name: Akihiko
full_name: Nakano, Akihiko
last_name: Nakano
- first_name: Marc
full_name: Van Montagu, Marc C
last_name: Van Montagu
- first_name: Hiroo
full_name: Fukuda, Hiroo
last_name: Fukuda
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Naramoto S, Kleine Vehn J, Robert S, et al. ADP ribosylation factor machinery
mediates endocytosis in plant cells. PNAS. 2010;107(50):21890-21895. doi:10.1073/pnas.1016260107
apa: Naramoto, S., Kleine Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek,
T., … Friml, J. (2010). ADP ribosylation factor machinery mediates endocytosis
in plant cells. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1016260107
chicago: Naramoto, Satoshi, Jürgen Kleine Vehn, Stéphanie Robert, Masaru Fujimoto,
Tomoko Dainobu, Tomasz Paciorek, Takashi Ueda, et al. “ADP Ribosylation Factor
Machinery Mediates Endocytosis in Plant Cells.” PNAS. National Academy
of Sciences, 2010. https://doi.org/10.1073/pnas.1016260107.
ieee: S. Naramoto et al., “ADP ribosylation factor machinery mediates endocytosis
in plant cells,” PNAS, vol. 107, no. 50. National Academy of Sciences,
pp. 21890–21895, 2010.
ista: Naramoto S, Kleine Vehn J, Robert S, Fujimoto M, Dainobu T, Paciorek T, Ueda
T, Nakano A, Van Montagu M, Fukuda H, Friml J. 2010. ADP ribosylation factor machinery
mediates endocytosis in plant cells. PNAS. 107(50), 21890–21895.
mla: Naramoto, Satoshi, et al. “ADP Ribosylation Factor Machinery Mediates Endocytosis
in Plant Cells.” PNAS, vol. 107, no. 50, National Academy of Sciences,
2010, pp. 21890–95, doi:10.1073/pnas.1016260107.
short: S. Naramoto, J. Kleine Vehn, S. Robert, M. Fujimoto, T. Dainobu, T. Paciorek,
T. Ueda, A. Nakano, M. Van Montagu, H. Fukuda, J. Friml, PNAS 107 (2010) 21890–21895.
date_created: 2018-12-11T12:01:15Z
date_published: 2010-12-14T00:00:00Z
date_updated: 2021-01-12T07:40:55Z
day: '14'
doi: 10.1073/pnas.1016260107
extern: 1
intvolume: ' 107'
issue: '50'
month: '12'
page: 21890 - 21895
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3621'
quality_controlled: 0
status: public
title: ADP ribosylation factor machinery mediates endocytosis in plant cells
type: journal_article
volume: 107
year: '2010'
...