---
_id: '3141'
abstract:
- lang: eng
  text: The two actin-related subunits of the Arp2/3 complex, Arp2 and Arp3, are proposed
    to form a pseudo actin dimer that nucleates actin polymerization. However, in
    the crystal structure of the inactive complex, they are too far apart to form
    such a nucleus. Here, we show using EM that yeast and bovine Arp2/3 complexes
    exist in a distribution among open, intermediate and closed conformations. The
    crystal structure docks well into the open conformation. The activator WASp binds
    at the cleft between Arp2 and Arp3, and all WASp-bound complexes are closed. The
    inhibitor coronin binds near the p35 subunit, and all coronin-bound complexes
    are open. Activating and loss-of-function mutations in the p35 subunit skew conformational
    distribution in opposite directions, closed and open, respectively. We conclude
    that WASp stabilizes p35-dependent closure of the complex, holding Arp2 and Arp3
    closer together to nucleate an actin filament.
author:
- first_name: Avital
  full_name: Rodal, Avital A
  last_name: Rodal
- first_name: Olga
  full_name: Sokolova, Olga
  last_name: Sokolova
- first_name: Deborah
  full_name: Robins, Deborah B
  last_name: Robins
- first_name: Karen
  full_name: Daugherty, Karen M
  last_name: Daugherty
- first_name: Simon
  full_name: Simon Hippenmeyer
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Howard
  full_name: Riezman, Howard
  last_name: Riezman
- first_name: Nikolaus
  full_name: Grigorieff, Nikolaus
  last_name: Grigorieff
- first_name: Bruce
  full_name: Goode, Bruce L
  last_name: Goode
citation:
  ama: Rodal A, Sokolova O, Robins D, et al. Conformational changes in the Arp2 3
    complex leading to actin nucleation. <i>Nature Structural and Molecular Biology</i>.
    2005;12(1):26-31. doi:<a href="https://doi.org/10.1038/nsmb870">10.1038/nsmb870</a>
  apa: Rodal, A., Sokolova, O., Robins, D., Daugherty, K., Hippenmeyer, S., Riezman,
    H., … Goode, B. (2005). Conformational changes in the Arp2 3 complex leading to
    actin nucleation. <i>Nature Structural and Molecular Biology</i>. Nature Publishing
    Group. <a href="https://doi.org/10.1038/nsmb870">https://doi.org/10.1038/nsmb870</a>
  chicago: Rodal, Avital, Olga Sokolova, Deborah Robins, Karen Daugherty, Simon Hippenmeyer,
    Howard Riezman, Nikolaus Grigorieff, and Bruce Goode. “Conformational Changes
    in the Arp2 3 Complex Leading to Actin Nucleation.” <i>Nature Structural and Molecular
    Biology</i>. Nature Publishing Group, 2005. <a href="https://doi.org/10.1038/nsmb870">https://doi.org/10.1038/nsmb870</a>.
  ieee: A. Rodal <i>et al.</i>, “Conformational changes in the Arp2 3 complex leading
    to actin nucleation,” <i>Nature Structural and Molecular Biology</i>, vol. 12,
    no. 1. Nature Publishing Group, pp. 26–31, 2005.
  ista: Rodal A, Sokolova O, Robins D, Daugherty K, Hippenmeyer S, Riezman H, Grigorieff
    N, Goode B. 2005. Conformational changes in the Arp2 3 complex leading to actin
    nucleation. Nature Structural and Molecular Biology. 12(1), 26–31.
  mla: Rodal, Avital, et al. “Conformational Changes in the Arp2 3 Complex Leading
    to Actin Nucleation.” <i>Nature Structural and Molecular Biology</i>, vol. 12,
    no. 1, Nature Publishing Group, 2005, pp. 26–31, doi:<a href="https://doi.org/10.1038/nsmb870">10.1038/nsmb870</a>.
  short: A. Rodal, O. Sokolova, D. Robins, K. Daugherty, S. Hippenmeyer, H. Riezman,
    N. Grigorieff, B. Goode, Nature Structural and Molecular Biology 12 (2005) 26–31.
date_created: 2018-12-11T12:01:38Z
date_published: 2005-01-01T00:00:00Z
date_updated: 2021-01-12T07:41:21Z
day: '01'
doi: 10.1038/nsmb870
extern: 1
intvolume: '        12'
issue: '1'
month: '01'
page: 26 - 31
publication: Nature Structural and Molecular Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3554'
quality_controlled: 0
status: public
title: Conformational changes in the Arp2 3 complex leading to actin nucleation
type: journal_article
volume: 12
year: '2005'
...