---
_id: '3150'
abstract:
- lang: eng
text: Tripartite G-protein-coupled receptors (GPCRs) represent one of the largest
groups of signal transducers, transmitting signals from hormones, neuropeptides,
odorants, food and light. Ligand-bound receptors catalyse GDP/GTP exchange on
the G-protein α-subunit (Gα), leading to α-GTP separation from the βγ subunits
and pathway activation. Activating mutations in the receptors or G proteins underlie
many human diseases, including some cancers, dwarfism and premature puberty. Regulators
of G-protein signalling (RGS proteins) are known to modulate the level and duration
of ligand-induced signalling by accelerating the intrinsic GTPase activity of
the Gα subunit, and thus reformation of the inactive GDP-bound Gα. Here we find
that even in the absence of receptor, mutation of the RGS family member Sst2 (refs
6-9) permits spontaneous activation of the G-protein-coupled mating pathway in
Saccharomyces cerevisiae at levels normally seen only in the presence of ligand.
Our work demonstrates the occurence of spontaneous tripartite G-protein signalling
in vivo and identifies a requirement for RGS proteins in preventing such receptor-independent
activation.
author:
- first_name: Daria E
full_name: Daria Siekhaus
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: David
full_name: Drubin, David G
last_name: Drubin
citation:
ama: Siekhaus DE, Drubin D. Spontaneous receptor-independent heterotrimeric G-protein
signalling in an RGS mutant. Nature Cell Biology. 2003;5(3):231-235. doi:10.1038/ncb941
apa: Siekhaus, D. E., & Drubin, D. (2003). Spontaneous receptor-independent
heterotrimeric G-protein signalling in an RGS mutant. Nature Cell Biology.
Nature Publishing Group. https://doi.org/10.1038/ncb941
chicago: Siekhaus, Daria E, and David Drubin. “Spontaneous Receptor-Independent
Heterotrimeric G-Protein Signalling in an RGS Mutant.” Nature Cell Biology.
Nature Publishing Group, 2003. https://doi.org/10.1038/ncb941.
ieee: D. E. Siekhaus and D. Drubin, “Spontaneous receptor-independent heterotrimeric
G-protein signalling in an RGS mutant,” Nature Cell Biology, vol. 5, no.
3. Nature Publishing Group, pp. 231–235, 2003.
ista: Siekhaus DE, Drubin D. 2003. Spontaneous receptor-independent heterotrimeric
G-protein signalling in an RGS mutant. Nature Cell Biology. 5(3), 231–235.
mla: Siekhaus, Daria E., and David Drubin. “Spontaneous Receptor-Independent Heterotrimeric
G-Protein Signalling in an RGS Mutant.” Nature Cell Biology, vol. 5, no.
3, Nature Publishing Group, 2003, pp. 231–35, doi:10.1038/ncb941.
short: D.E. Siekhaus, D. Drubin, Nature Cell Biology 5 (2003) 231–235.
date_created: 2018-12-11T12:01:41Z
date_published: 2003-03-01T00:00:00Z
date_updated: 2021-01-12T07:41:24Z
day: '01'
doi: 10.1038/ncb941
extern: 1
intvolume: ' 5'
issue: '3'
month: '03'
page: 231 - 235
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3544'
quality_controlled: 0
status: public
title: Spontaneous receptor-independent heterotrimeric G-protein signalling in an
RGS mutant
type: journal_article
volume: 5
year: '2003'
...