--- res: bibo_abstract: - The functional role of AMPA receptor (AMPAR)-mediated synaptic signaling between neurons and oligodendrocyte precursor cells (OPCs) remains enigmatic. We modified the properties of AMPARs at axon-OPC synapses in the mouse corpus callosum in vivo during the peak of myelination by targeting the GluA2 subunit. Expression of the unedited (Ca2+ permeable) or the pore-dead GluA2 subunit of AMPARs triggered proliferation of OPCs and reduced their differentiation into oligodendrocytes. Expression of the cytoplasmic C-terminal (GluA2(813-862)) of the GluA2 subunit (C-tail), a modification designed to affect the interaction between GluA2 and AMPAR-binding proteins and to perturb trafficking of GluA2-containing AMPARs, decreased the differentiation of OPCs without affecting their proliferation. These findings suggest that ionotropic and non-ionotropic properties of AMPARs in OPCs, as well as specific aspects of AMPAR-mediated signaling at axon-OPC synapses in the mouse corpus callosum, are important for balancing the response of OPCs to proliferation and differentiation cues. In the brain, oligodendrocyte precursor cells (OPCs) receive glutamatergic AMPA-receptor-mediated synaptic input from neurons. Chen et al. show that modifying AMPA-receptor properties at axon-OPC synapses alters proliferation and differentiation of OPCs. This expands the traditional view of synaptic transmission by suggesting neurons also use synapses to modulate behavior of glia.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Ting foaf_name: Chen, Ting foaf_surname: Chen - foaf_Person: foaf_givenName: Bartosz foaf_name: Kula, Bartosz foaf_surname: Kula - foaf_Person: foaf_givenName: Balint foaf_name: Nagy, Balint foaf_surname: Nagy foaf_workInfoHomepage: http://www.librecat.org/personId=30F830CE-02D1-11E9-9BAA-DAF4881429F2 orcid: 0000-0002-4002-4686 - foaf_Person: foaf_givenName: Ruxandra foaf_name: Barzan, Ruxandra foaf_surname: Barzan - foaf_Person: foaf_givenName: Andrea foaf_name: Gall, Andrea foaf_surname: Gall - foaf_Person: foaf_givenName: Ingrid foaf_name: Ehrlich, Ingrid foaf_surname: Ehrlich - foaf_Person: foaf_givenName: Maria foaf_name: Kukley, Maria foaf_surname: Kukley bibo_doi: 10.1016/j.celrep.2018.09.066 bibo_issue: '4' bibo_volume: 25 dct_date: 2018^xs_gYear dct_identifier: - UT:000448219500005 dct_language: eng dct_publisher: Elsevier@ dct_title: In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2@ ...