---
_id: '3376'
abstract:
- lang: eng
  text: Regulatory conflicts occur when two signals that individually trigger opposite
    cellular responses are present simultaneously. Here, we investigate regulatory
    conflicts in the bacterial response to antibiotic combinations. We use an Escherichia
    coli promoter-GFP library to study the transcriptional response of many promoters
    to either additive or antagonistic drug pairs at fine two-dimensional (2D) resolution
    of drug concentration. Surprisingly, we find that this data set can be characterized
    as a linear sum of only two principal components. Component one, accounting for
    over 70% of the response, represents the response to growth inhibition by the
    drugs. Component two describes how regulatory conflicts are resolved. For the
    additive drug pair, conflicts are resolved by linearly interpolating the single
    drug responses, while for the antagonistic drug pair, the growth-limiting drug
    dominates the response. Importantly, for a given drug pair, the same conflict
    resolution strategy applies to almost all genes. These results provide a recipe
    for predicting gene expression responses to antibiotic combinations.
acknowledgement: This work was supported by a Feodor Lynen Fellowship of the Alexander
  von Humboldt Foundation (to T.B.).
article_processing_charge: No
author:
- first_name: Mark Tobias
  full_name: Bollenbach, Mark Tobias
  id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
  last_name: Bollenbach
  orcid: 0000-0003-4398-476X
- first_name: Roy
  full_name: Kishony, Roy
  last_name: Kishony
citation:
  ama: Bollenbach MT, Kishony R. Resolution of gene regulatory conflicts caused by
    combinations of antibiotics. <i>Molecular Cell</i>. 2011;42(4):413-425. doi:<a
    href="https://doi.org/10.1016/j.molcel.2011.04.016">10.1016/j.molcel.2011.04.016</a>
  apa: Bollenbach, M. T., &#38; Kishony, R. (2011). Resolution of gene regulatory
    conflicts caused by combinations of antibiotics. <i>Molecular Cell</i>. Cell Press.
    <a href="https://doi.org/10.1016/j.molcel.2011.04.016">https://doi.org/10.1016/j.molcel.2011.04.016</a>
  chicago: Bollenbach, Mark Tobias, and Roy Kishony. “Resolution of Gene Regulatory
    Conflicts Caused by Combinations of Antibiotics.” <i>Molecular Cell</i>. Cell
    Press, 2011. <a href="https://doi.org/10.1016/j.molcel.2011.04.016">https://doi.org/10.1016/j.molcel.2011.04.016</a>.
  ieee: M. T. Bollenbach and R. Kishony, “Resolution of gene regulatory conflicts
    caused by combinations of antibiotics,” <i>Molecular Cell</i>, vol. 42, no. 4.
    Cell Press, pp. 413–425, 2011.
  ista: Bollenbach MT, Kishony R. 2011. Resolution of gene regulatory conflicts caused
    by combinations of antibiotics. Molecular Cell. 42(4), 413–425.
  mla: Bollenbach, Mark Tobias, and Roy Kishony. “Resolution of Gene Regulatory Conflicts
    Caused by Combinations of Antibiotics.” <i>Molecular Cell</i>, vol. 42, no. 4,
    Cell Press, 2011, pp. 413–25, doi:<a href="https://doi.org/10.1016/j.molcel.2011.04.016">10.1016/j.molcel.2011.04.016</a>.
  short: M.T. Bollenbach, R. Kishony, Molecular Cell 42 (2011) 413–425.
date_created: 2018-12-11T12:02:59Z
date_published: 2011-05-20T00:00:00Z
date_updated: 2025-09-30T08:54:28Z
day: '20'
department:
- _id: ToBo
doi: 10.1016/j.molcel.2011.04.016
external_id:
  isi:
  - '000291113800005'
fulldoi: https://doi.org/10.1016/j.molcel.2011.04.016
intvolume: '        42'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3143497/
month: '05'
oa: 1
oa_version: Submitted Version
page: 413 - 425
publication: Molecular Cell
publication_status: published
publisher: Cell Press
publist_id: '3231'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Resolution of gene regulatory conflicts caused by combinations of antibiotics
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 42
year: '2011'
...