---
OA_place: repository
OA_type: green
_id: '3379'
abstract:
- lang: eng
  text: The process of gastrulation is highly conserved across vertebrates on both
    the genetic and morphological levels, despite great variety in embryonic shape
    and speed of development. This mechanism spatially separates the germ layers and
    establishes the organizational foundation for future development. Mesodermal identity
    is specified in a superficial layer of cells, the epiblast, where cells maintain
    an epithelioid morphology. These cells involute to join the deeper hypoblast layer
    where they adopt a migratory, mesenchymal morphology. Expression of a cascade
    of related transcription factors orchestrates the parallel genetic transition
    from primitive to mature mesoderm. Although the early and late stages of this
    process are increasingly well understood, the transition between them has remained
    largely mysterious. We present here the first high resolution in vivo observations
    of the blebby transitional morphology of involuting mesodermal cells in a vertebrate
    embryo. We further demonstrate that the zebrafish spadetail mutation creates a
    reversible block in the maturation program, stalling cells in the transition state.
    This mutation creates an ideal system for dissecting the specific properties of
    cells undergoing the morphological transition of maturing mesoderm, as we demonstrate
    with a direct measurement of cell–cell adhesion.
acknowledgement: We thank David Grunwald for providing the spadetail-myc fusion construct.
  This work was supported by an NIH grant (GM079203) to D.K. and grants from the Austrian
  Academy of Sciences to P.S., and from the DFG, MPG and IST Austria to C.-P.H. R.R.
  was supported by a Developmental Biology Predoctoral Training Grant, T32HD007183,
  from the National Institute of Child Health and Human Development. B.L.M. was supported
  by an American Cancer Society fellowship (PF-07-048-01-DDC).
article_processing_charge: No
article_type: original
author:
- first_name: Richard
  full_name: Row, Richard
  last_name: Row
- first_name: Jean-Léon
  full_name: Maître, Jean-Léon
  id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
  last_name: Maître
  orcid: 0000-0002-3688-1474
- first_name: Benjamin
  full_name: Martin, Benjamin
  last_name: Martin
- first_name: Petra
  full_name: Stockinger, Petra
  id: 261CB030-E90D-11E9-B182-F697D44B663C
  last_name: Stockinger
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: David
  full_name: Kimelman, David
  last_name: Kimelman
citation:
  ama: Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. Completion
    of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail.
    <i>Developmental Biology</i>. 2011;354(1):102-110. doi:<a href="https://doi.org/10.1016/j.ydbio.2011.03.025">10.1016/j.ydbio.2011.03.025</a>
  apa: Row, R., Maître, J.-L., Martin, B., Stockinger, P., Heisenberg, C.-P. J., &#38;
    Kimelman, D. (2011). Completion of the epithelial to mesenchymal transition in
    zebrafish mesoderm requires Spadetail. <i>Developmental Biology</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.ydbio.2011.03.025">https://doi.org/10.1016/j.ydbio.2011.03.025</a>
  chicago: Row, Richard, Jean-Léon Maître, Benjamin Martin, Petra Stockinger, Carl-Philipp
    J Heisenberg, and David Kimelman. “Completion of the Epithelial to Mesenchymal
    Transition in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>.
    Elsevier, 2011. <a href="https://doi.org/10.1016/j.ydbio.2011.03.025">https://doi.org/10.1016/j.ydbio.2011.03.025</a>.
  ieee: R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P. J. Heisenberg, and D.
    Kimelman, “Completion of the epithelial to mesenchymal transition in zebrafish
    mesoderm requires Spadetail,” <i>Developmental Biology</i>, vol. 354, no. 1. Elsevier,
    pp. 102–110, 2011.
  ista: Row R, Maître J-L, Martin B, Stockinger P, Heisenberg C-PJ, Kimelman D. 2011.
    Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires
    Spadetail. Developmental Biology. 354(1), 102–110.
  mla: Row, Richard, et al. “Completion of the Epithelial to Mesenchymal Transition
    in Zebrafish Mesoderm Requires Spadetail.” <i>Developmental Biology</i>, vol.
    354, no. 1, Elsevier, 2011, pp. 102–10, doi:<a href="https://doi.org/10.1016/j.ydbio.2011.03.025">10.1016/j.ydbio.2011.03.025</a>.
  short: R. Row, J.-L. Maître, B. Martin, P. Stockinger, C.-P.J. Heisenberg, D. Kimelman,
    Developmental Biology 354 (2011) 102–110.
das_tickbox: '1'
date_created: 2018-12-11T12:03:00Z
date_published: 2011-06-01T00:00:00Z
date_updated: 2026-07-28T08:31:27Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/j.ydbio.2011.03.025
external_id:
  isi:
  - '000290550500010'
  pmid:
  - '21463614'
fulldoi: https://doi.org/10.1016/j.ydbio.2011.03.025
intvolume: '       354'
isi: 1
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090540/
month: '06'
oa: 1
oa_version: Submitted Version
page: 102 - 110
pmid: 1
publication: Developmental Biology
publication_status: published
publisher: Elsevier
publist_id: '3228'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Completion of the epithelial to mesenchymal transition in zebrafish mesoderm
  requires Spadetail
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 354
year: '2011'
...