---
_id: '3407'
abstract:
- lang: eng
  text: Genetically targeted light-activated ion channels and pumps make it possible
    to determine the role of specific neurons in neuronal circuits, information processing
    and behavior. We developed a K+-selective ionotropic glutamate receptor that reversibly
    inhibits neuronal activity in response to light in dissociated neurons and brain
    slice and also reversibly suppresses behavior in zebrafish. The receptor is a
    chimera of the pore region of a K+-selective bacterial glutamate receptor and
    the ligand-binding domain of a light-gated mammalian kainate receptor. This hyperpolarizing
    light-gated channel, HyLighter, is turned on by a brief light pulse at one wavelength
    and turned off by a pulse at a second wavelength. The control is obtained at moderate
    intensity. After optical activation, the photocurrent and optical silencing of
    activity persists in the dark for extended periods. The low light requirement
    and bi-stability of HyLighter represent advantages for the dissection of neural
    circuitry.
author:
- first_name: Harald L
  full_name: Harald Janovjak
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
- first_name: Stephanie
  full_name: Szobota, Stephanie
  last_name: Szobota
- first_name: Claire
  full_name: Wyart, Claire
  last_name: Wyart
- first_name: Dirk
  full_name: Trauner, Dirk
  last_name: Trauner
- first_name: Ehud
  full_name: Isacoff, Ehud Y
  last_name: Isacoff
citation:
  ama: Janovjak HL, Szobota S, Wyart C, Trauner D, Isacoff E. A light-gated, potassium-selective
    glutamate receptor for the optical inhibition of neuronal firing. <i>Nature Neuroscience</i>.
    2010;13:1027-1032. doi:<a href="https://doi.org/10.1038/nn.2589">10.1038/nn.2589</a>
  apa: Janovjak, H. L., Szobota, S., Wyart, C., Trauner, D., &#38; Isacoff, E. (2010).
    A light-gated, potassium-selective glutamate receptor for the optical inhibition
    of neuronal firing. <i>Nature Neuroscience</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nn.2589">https://doi.org/10.1038/nn.2589</a>
  chicago: Janovjak, Harald L, Stephanie Szobota, Claire Wyart, Dirk Trauner, and
    Ehud Isacoff. “A Light-Gated, Potassium-Selective Glutamate Receptor for the Optical
    Inhibition of Neuronal Firing.” <i>Nature Neuroscience</i>. Nature Publishing
    Group, 2010. <a href="https://doi.org/10.1038/nn.2589">https://doi.org/10.1038/nn.2589</a>.
  ieee: H. L. Janovjak, S. Szobota, C. Wyart, D. Trauner, and E. Isacoff, “A light-gated,
    potassium-selective glutamate receptor for the optical inhibition of neuronal
    firing,” <i>Nature Neuroscience</i>, vol. 13. Nature Publishing Group, pp. 1027–1032,
    2010.
  ista: Janovjak HL, Szobota S, Wyart C, Trauner D, Isacoff E. 2010. A light-gated,
    potassium-selective glutamate receptor for the optical inhibition of neuronal
    firing. Nature Neuroscience. 13, 1027–1032.
  mla: Janovjak, Harald L., et al. “A Light-Gated, Potassium-Selective Glutamate Receptor
    for the Optical Inhibition of Neuronal Firing.” <i>Nature Neuroscience</i>, vol.
    13, Nature Publishing Group, 2010, pp. 1027–32, doi:<a href="https://doi.org/10.1038/nn.2589">10.1038/nn.2589</a>.
  short: H.L. Janovjak, S. Szobota, C. Wyart, D. Trauner, E. Isacoff, Nature Neuroscience
    13 (2010) 1027–1032.
date_created: 2018-12-11T12:03:10Z
date_published: 2010-06-27T00:00:00Z
date_updated: 2021-01-12T07:43:16Z
day: '27'
doi: 10.1038/nn.2589
extern: 1
intvolume: '        13'
month: '06'
page: 1027 - 1032
publication: Nature Neuroscience
publication_status: published
publisher: Nature Publishing Group
publist_id: '2995'
quality_controlled: 0
status: public
title: A light-gated, potassium-selective glutamate receptor for the optical inhibition
  of neuronal firing
type: journal_article
volume: 13
year: '2010'
...