---
_id: '3409'
abstract:
- lang: eng
  text: With the introduction of single-molecule force spectroscopy (SMFS) it has
    become possible to directly access the interactions of various molecular systems.
    A bottleneck in conventional SMFS is collecting the large amount of data required
    for statistically meaningful analysis. Currently, atomic force microscopy (AFM)-based
    SMFS requires the user to tediously 'fish' for single molecules. In addition,
    most experimental and environmental conditions must be manually adjusted. Here,
    we developed a fully automated single-molecule force spectroscope. The instrument
    is able to perform SMFS while monitoring and regulating experimental conditions
    such as buffer composition and temperature. Cantilever alignment and calibration
    can also be automatically performed during experiments. This, combined with in-line
    data analysis, enables the instrument, once set up, to perform complete SMFS experiments
    autonomously.
author:
- first_name: Jens
  full_name: Struckmeier, Jens
  last_name: Struckmeier
- first_name: Reiner
  full_name: Wahl, Reiner
  last_name: Wahl
- first_name: Mirko
  full_name: Leuschner, Mirko
  last_name: Leuschner
- first_name: Joao
  full_name: Nunes, Joao
  last_name: Nunes
- first_name: Harald L
  full_name: Harald Janovjak
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
- first_name: Ulrich
  full_name: Geisler, Ulrich
  last_name: Geisler
- first_name: Gerd
  full_name: Hofmann, Gerd
  last_name: Hofmann
- first_name: Torsten
  full_name: Jähnke, Torsten
  last_name: Jähnke
- first_name: Daniel
  full_name: Mueller, Daniel J
  last_name: Mueller
citation:
  ama: Struckmeier J, Wahl R, Leuschner M, et al. Fully automated single-molecule
    force spectroscopy for screening applications. <i>Nanotechnology</i>. 2008;19(38).
    doi:<a href="https://doi.org/10.1088/0957-4484/19/38/384020">10.1088/0957-4484/19/38/384020</a>
  apa: Struckmeier, J., Wahl, R., Leuschner, M., Nunes, J., Janovjak, H. L., Geisler,
    U., … Mueller, D. (2008). Fully automated single-molecule force spectroscopy for
    screening applications. <i>Nanotechnology</i>. IOP Publishing Ltd. <a href="https://doi.org/10.1088/0957-4484/19/38/384020">https://doi.org/10.1088/0957-4484/19/38/384020</a>
  chicago: Struckmeier, Jens, Reiner Wahl, Mirko Leuschner, Joao Nunes, Harald L Janovjak,
    Ulrich Geisler, Gerd Hofmann, Torsten Jähnke, and Daniel Mueller. “Fully Automated
    Single-Molecule Force Spectroscopy for Screening Applications.” <i>Nanotechnology</i>.
    IOP Publishing Ltd., 2008. <a href="https://doi.org/10.1088/0957-4484/19/38/384020">https://doi.org/10.1088/0957-4484/19/38/384020</a>.
  ieee: J. Struckmeier <i>et al.</i>, “Fully automated single-molecule force spectroscopy
    for screening applications,” <i>Nanotechnology</i>, vol. 19, no. 38. IOP Publishing
    Ltd., 2008.
  ista: Struckmeier J, Wahl R, Leuschner M, Nunes J, Janovjak HL, Geisler U, Hofmann
    G, Jähnke T, Mueller D. 2008. Fully automated single-molecule force spectroscopy
    for screening applications. Nanotechnology. 19(38).
  mla: Struckmeier, Jens, et al. “Fully Automated Single-Molecule Force Spectroscopy
    for Screening Applications.” <i>Nanotechnology</i>, vol. 19, no. 38, IOP Publishing
    Ltd., 2008, doi:<a href="https://doi.org/10.1088/0957-4484/19/38/384020">10.1088/0957-4484/19/38/384020</a>.
  short: J. Struckmeier, R. Wahl, M. Leuschner, J. Nunes, H.L. Janovjak, U. Geisler,
    G. Hofmann, T. Jähnke, D. Mueller, Nanotechnology 19 (2008).
date_created: 2018-12-11T12:03:11Z
date_published: 2008-08-12T00:00:00Z
date_updated: 2021-01-12T07:43:17Z
day: '12'
doi: 10.1088/0957-4484/19/38/384020
extern: 1
intvolume: '        19'
issue: '38'
month: '08'
publication: Nanotechnology
publication_status: published
publisher: IOP Publishing Ltd.
publist_id: '2993'
quality_controlled: 0
status: public
title: Fully automated single-molecule force spectroscopy for screening applications
type: journal_article
volume: 19
year: '2008'
...