---
_id: '3412'
abstract:
- lang: eng
text: |-
Molecular interactions are the basic language of biological processes.
They establish the forces interacting between the building blocks of
proteins and other macromolecules, thus determining their functional
roles. Because molecular interactions trigger virtually every
biological process, approaches to decipher their language are needed.
Single-molecule force spectroscopy (SMFS) has been used to detect
and characterize different types of molecular interactions that occur
between and within native membrane proteins. The first experiments
detected and localized molecular interactions that stabilized
membrane proteins, including how these interactions were established
during folding of α-helical secondary structure elements into
the native protein and how they changed with oligomerization, temperature,
and mutations. SMFS also enables investigators to detect
and locate molecular interactions established during ligand and inhibitor
binding. These exciting applications provide opportunities
for studying the molecular forces of life. Further developments will
elucidate the origins of molecular interactions encoded in their lifetimes,
interaction ranges, interplay, and dynamics characteristic of biological systems.
author:
- first_name: Alexej
full_name: Kedrov, Alexej
last_name: Kedrov
- first_name: Harald L
full_name: Harald Janovjak
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Tanuj
full_name: Sapra, Tanuj K
last_name: Sapra
- first_name: Daniel
full_name: Mueller, Daniel J
last_name: Mueller
citation:
ama: Kedrov A, Janovjak HL, Sapra T, Mueller D. Deciphering molecular interactions
of native membrane proteins by single-molecule force spectroscopy. Annual Review
of Biophysics. 2007;36:233-260. doi:10.1146/annurev.biophys.36.040306.132640
apa: Kedrov, A., Janovjak, H. L., Sapra, T., & Mueller, D. (2007). Deciphering
molecular interactions of native membrane proteins by single-molecule force spectroscopy.
Annual Review of Biophysics. Annual Reviews. https://doi.org/10.1146/annurev.biophys.36.040306.132640
chicago: Kedrov, Alexej, Harald L Janovjak, Tanuj Sapra, and Daniel Mueller. “Deciphering
Molecular Interactions of Native Membrane Proteins by Single-Molecule Force Spectroscopy.”
Annual Review of Biophysics. Annual Reviews, 2007. https://doi.org/10.1146/annurev.biophys.36.040306.132640.
ieee: A. Kedrov, H. L. Janovjak, T. Sapra, and D. Mueller, “Deciphering molecular
interactions of native membrane proteins by single-molecule force spectroscopy,”
Annual Review of Biophysics, vol. 36. Annual Reviews, pp. 233–260, 2007.
ista: Kedrov A, Janovjak HL, Sapra T, Mueller D. 2007. Deciphering molecular interactions
of native membrane proteins by single-molecule force spectroscopy. Annual Review
of Biophysics. 36, 233–260.
mla: Kedrov, Alexej, et al. “Deciphering Molecular Interactions of Native Membrane
Proteins by Single-Molecule Force Spectroscopy.” Annual Review of Biophysics,
vol. 36, Annual Reviews, 2007, pp. 233–60, doi:10.1146/annurev.biophys.36.040306.132640.
short: A. Kedrov, H.L. Janovjak, T. Sapra, D. Mueller, Annual Review of Biophysics
36 (2007) 233–260.
date_created: 2018-12-11T12:03:11Z
date_published: 2007-06-01T00:00:00Z
date_updated: 2019-04-26T07:22:27Z
day: '01'
doi: 10.1146/annurev.biophys.36.040306.132640
extern: 1
intvolume: ' 36'
month: '06'
page: 233 - 260
publication: Annual Review of Biophysics
publication_status: published
publisher: Annual Reviews
publist_id: '2989'
quality_controlled: 0
status: public
title: Deciphering molecular interactions of native membrane proteins by single-molecule
force spectroscopy
type: review
volume: 36
year: '2007'
...