---
_id: '3489'
abstract:
- lang: eng
  text: We have examined factors that determine the strength and dynamics of GABAergic
    synapses between interneurons [dentate gyrus basket cells (BCs)] and principal
    neurons [dentate gyrus granule cells (GCs)] using paired recordings in rat hippocampal
    slices at 34°C. Unitary IPSCs recorded from BC–GC pairs in high intracellular
    Cl− concentration showed a fast rise and a biexponential decay, with mean time
    constants of 2 and 9 msec. The mean quantal conductance change, determined directly
    at reduced extracellular Ca2+/Mg2+concentration ratios, was 1.7 nS. Quantal release
    at the BC–GC synapse occurred with short delay and was highly synchronized. Analysis
    of IPSC peak amplitudes and numbers of failures by multiple probability compound
    binomial analysis indicated that synaptic transmission at the BC–GC synapse involves
    three to seven release sites, each of which releases transmitter with high probability
    (∼0.5 in 2 mMCa2+/1 mM Mg2+). Unitary BC–GC IPSCs showed paired-pulse depression
    (PPD); maximal depression, measured for 10 msec intervals, was 37%, and recovery
    from depression occurred with a time constant of 2 sec. Paired-pulse depression
    was mainly presynaptic in origin but appeared to be independent of previous release.
    Synaptic transmission at the BC–GC synapse showed frequency-dependent depression,
    with half-maximal decrease at 5 Hz after a series of 1000 presynaptic action potentials.
    The relative stability of transmission at the BC–GC synapse is consistent with
    a model in which an activity-dependent gating mechanism reduces release probability
    and thereby prevents depletion of the releasable pool of synaptic vesicles. Thus
    several mechanisms converge on the generation of powerful and sustained transmission
    at interneuron–principal neuron synapses in hippocampal circuits.
acknowledgement: This work was supported by grants from the Deutsche Forschungsgemeinschaft
  (SFB 505/C5) and the Human Frontiers Science Program Organization (RG0017/1998-B)
  to P.J. Novartis generously provided CGP55845A. We thank Drs. J. Bischofberger,
  F. A. Edwards, J. R. P. Geiger, M. V. Jones, M. Martina, and A. Roth for critically
  reading this manuscript. We also thank A. Blomenkamp for technical assistance.
article_processing_charge: No
article_type: original
author:
- first_name: Udo
  full_name: Kraushaar, Udo
  last_name: Kraushaar
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Kraushaar U, Jonas PM. Efficacy and stability of quantal GABA release at a
    hippocampal interneuron-principal neuron synapse. <i>Journal of Neuroscience</i>.
    2000;20(15):5594-5607. doi:<a href="https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000">10.1523/JNEUROSCI.20-15-05594.2000</a>
  apa: Kraushaar, U., &#38; Jonas, P. M. (2000). Efficacy and stability of quantal
    GABA release at a hippocampal interneuron-principal neuron synapse. <i>Journal
    of Neuroscience</i>. Society for Neuroscience. <a href="https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000">https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000</a>
  chicago: Kraushaar, Udo, and Peter M Jonas. “Efficacy and Stability of Quantal GABA
    Release at a Hippocampal Interneuron-Principal Neuron Synapse.” <i>Journal of
    Neuroscience</i>. Society for Neuroscience, 2000. <a href="https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000">https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000</a>.
  ieee: U. Kraushaar and P. M. Jonas, “Efficacy and stability of quantal GABA release
    at a hippocampal interneuron-principal neuron synapse,” <i>Journal of Neuroscience</i>,
    vol. 20, no. 15. Society for Neuroscience, pp. 5594–5607, 2000.
  ista: Kraushaar U, Jonas PM. 2000. Efficacy and stability of quantal GABA release
    at a hippocampal interneuron-principal neuron synapse. Journal of Neuroscience.
    20(15), 5594–5607.
  mla: Kraushaar, Udo, and Peter M. Jonas. “Efficacy and Stability of Quantal GABA
    Release at a Hippocampal Interneuron-Principal Neuron Synapse.” <i>Journal of
    Neuroscience</i>, vol. 20, no. 15, Society for Neuroscience, 2000, pp. 5594–607,
    doi:<a href="https://doi.org/10.1523/JNEUROSCI.20-15-05594.2000">10.1523/JNEUROSCI.20-15-05594.2000</a>.
  short: U. Kraushaar, P.M. Jonas, Journal of Neuroscience 20 (2000) 5594–5607.
date_created: 2018-12-11T12:03:36Z
date_published: 2000-08-01T00:00:00Z
date_updated: 2023-05-03T08:18:39Z
day: '01'
doi: 10.1523/JNEUROSCI.20-15-05594.2000
extern: '1'
external_id:
  pmid:
  - '10908596'
intvolume: '        20'
issue: '15'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6772523/
month: '08'
oa: 1
oa_version: Published Version
page: 5594 - 5607
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
  issn:
  - 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '2898'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Efficacy and stability of quantal GABA release at a hippocampal interneuron-principal
  neuron synapse
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 20
year: '2000'
...