---
res:
  bibo_abstract:
  - The use of advanced patch-clamp recording techniques in brain slices, such as
    simultaneous recording from multiple neurons and recording from dendrites or presynaptic
    terminals, demands slices of the highest quality. In this context the mechanics
    of the tissue slicer are an important factor. Ideally, a tissue slicer should
    generate large-amplitude and high-frequency movements of the cutting blade in
    a horizontal axis, with minimal vibrations in the vertical axis. We developed
    a vibroslicer that fulfils these in part conflicting requirements. The oscillator
    is a permanent-magnet-coil-leaf-spring system. Using an auto-resonant mechano-electrical
    feedback circuit, large horizontal oscillations (up to 3 mm peak-to-peak) with
    high frequency (,90 Hz) are generated. To minimize vertical vibrations, an adjustment
    mechanism was employed that allowed alignment of the cutting edge of the blade
    with the major axis of the oscillation. A vibroprobe device was used to monitor
    vertical vibrations during adjustment. The system is based on the shading of the
    light path between a light-emitting diode (LED) and a photodiode. Vibroprobe monitoring
    revealed that the vibroslicer, after appropriate adjustment, generated vertical
    vibrations of <1 µm, significantly less than many commercial tissue slicers.
    Light- and electron-microscopic analysis of surface layers of slices cut with
    the vibroslicer showed that cellular elements, dendritic processes and presynaptic
    terminals are well preserved under these conditions, as required for patch-clamp
    recording from these structures.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Jörg
      foaf_name: Geiger, Jörg
      foaf_surname: Geiger
  - foaf_Person:
      foaf_givenName: Joseph
      foaf_name: Bischofberger, Joseph
      foaf_surname: Bischofberger
  - foaf_Person:
      foaf_givenName: Imre
      foaf_name: Vida, Imre
      foaf_surname: Vida
  - foaf_Person:
      foaf_givenName: Ulrich
      foaf_name: Fröbe, Ulrich
      foaf_surname: Fröbe
  - foaf_Person:
      foaf_givenName: S
      foaf_name: Pfitzinger, S
      foaf_surname: Pfitzinger
  - foaf_Person:
      foaf_givenName: H.
      foaf_name: Weber, H.
      foaf_surname: Weber
  - foaf_Person:
      foaf_givenName: Klaus
      foaf_name: Haverkampf, Klaus
      foaf_surname: Haverkampf
  - foaf_Person:
      foaf_givenName: Peter M
      foaf_name: Jonas, Peter M
      foaf_surname: Jonas
      foaf_workInfoHomepage: http://www.librecat.org/personId=353C1B58-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-5001-4804
  bibo_doi: 10.1007/s00424-001-0735-3
  bibo_issue: '3'
  bibo_volume: 443
  dct_date: 2002^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0031-6768
  dct_language: eng
  dct_publisher: Springer@
  dct_title: Patch-clamp recording in brain slices with improved slicer technology@
  fabio_hasPubmedId: '11810221'
...