---
res:
  bibo_abstract:
  - Developmental processes are inherently dynamic and understanding them requires
    quantitative measurements of gene and protein expression levels in space and time.
    While live imaging is a powerful approach for obtaining such data, it is still
    a challenge to apply it over long periods of time to large tissues, such as the
    embryonic spinal cord in mouse and chick. Nevertheless, dynamics of gene expression
    and signaling activity patterns in this organ can be studied by collecting tissue
    sections at different developmental stages. In combination with immunohistochemistry,
    this allows for measuring the levels of multiple developmental regulators in a
    quantitative manner with high spatiotemporal resolution. The mean protein expression
    levels over time, as well as embryo-to-embryo variability can be analyzed. A key
    aspect of the approach is the ability to compare protein levels across different
    samples. This requires a number of considerations in sample preparation, imaging
    and data analysis. Here we present a protocol for obtaining time course data of
    dorsoventral expression patterns from mouse and chick neural tube in the first
    3 days of neural tube development. The described workflow starts from embryo dissection
    and ends with a processed dataset. Software scripts for data analysis are included.
    The protocol is adaptable and instructions that allow the user to modify different
    steps are provided. Thus, the procedure can be altered for analysis of time-lapse
    images and applied to systems other than the neural tube.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Marcin P
      foaf_name: Zagórski, Marcin P
      foaf_surname: Zagórski
      foaf_workInfoHomepage: http://www.librecat.org/personId=343DA0DC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-7896-7762
  - foaf_Person:
      foaf_givenName: Anna
      foaf_name: Kicheva, Anna
      foaf_surname: Kicheva
      foaf_workInfoHomepage: http://www.librecat.org/personId=3959A2A0-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4509-4998
  bibo_doi: 10.1007/978-1-4939-8772-6_4
  bibo_volume: 1863
  dct_date: 2018^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/1064-3745
  - http://id.crossref.org/issn/978-1-4939-8771-9
  dct_language: eng
  dct_publisher: Springer Nature@
  dct_title: Measuring dorsoventral pattern and morphogen signaling profiles in the
    growing neural tube@
...