---
_id: '37'
abstract:
- lang: eng
  text: Developmental processes are inherently dynamic and understanding them requires
    quantitative measurements of gene and protein expression levels in space and time.
    While live imaging is a powerful approach for obtaining such data, it is still
    a challenge to apply it over long periods of time to large tissues, such as the
    embryonic spinal cord in mouse and chick. Nevertheless, dynamics of gene expression
    and signaling activity patterns in this organ can be studied by collecting tissue
    sections at different developmental stages. In combination with immunohistochemistry,
    this allows for measuring the levels of multiple developmental regulators in a
    quantitative manner with high spatiotemporal resolution. The mean protein expression
    levels over time, as well as embryo-to-embryo variability can be analyzed. A key
    aspect of the approach is the ability to compare protein levels across different
    samples. This requires a number of considerations in sample preparation, imaging
    and data analysis. Here we present a protocol for obtaining time course data of
    dorsoventral expression patterns from mouse and chick neural tube in the first
    3 days of neural tube development. The described workflow starts from embryo dissection
    and ends with a processed dataset. Software scripts for data analysis are included.
    The protocol is adaptable and instructions that allow the user to modify different
    steps are provided. Thus, the procedure can be altered for analysis of time-lapse
    images and applied to systems other than the neural tube.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Marcin P
  full_name: Zagórski, Marcin P
  id: 343DA0DC-F248-11E8-B48F-1D18A9856A87
  last_name: Zagórski
  orcid: 0000-0001-7896-7762
- first_name: Anna
  full_name: Kicheva, Anna
  id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
  last_name: Kicheva
  orcid: 0000-0003-4509-4998
citation:
  ama: 'Zagórski MP, Kicheva A. Measuring dorsoventral pattern and morphogen signaling
    profiles in the growing neural tube. In: <i>Morphogen Gradients </i>. Vol 1863.
    MIMB. Springer Nature; 2018:47-63. doi:<a href="https://doi.org/10.1007/978-1-4939-8772-6_4">10.1007/978-1-4939-8772-6_4</a>'
  apa: Zagórski, M. P., &#38; Kicheva, A. (2018). Measuring dorsoventral pattern and
    morphogen signaling profiles in the growing neural tube. In <i>Morphogen Gradients
    </i> (Vol. 1863, pp. 47–63). Springer Nature. <a href="https://doi.org/10.1007/978-1-4939-8772-6_4">https://doi.org/10.1007/978-1-4939-8772-6_4</a>
  chicago: Zagórski, Marcin P, and Anna Kicheva. “Measuring Dorsoventral Pattern and
    Morphogen Signaling Profiles in the Growing Neural Tube.” In <i>Morphogen Gradients
    </i>, 1863:47–63. MIMB. Springer Nature, 2018. <a href="https://doi.org/10.1007/978-1-4939-8772-6_4">https://doi.org/10.1007/978-1-4939-8772-6_4</a>.
  ieee: M. P. Zagórski and A. Kicheva, “Measuring dorsoventral pattern and morphogen
    signaling profiles in the growing neural tube,” in <i>Morphogen Gradients </i>,
    vol. 1863, Springer Nature, 2018, pp. 47–63.
  ista: 'Zagórski MP, Kicheva A. 2018.Measuring dorsoventral pattern and morphogen
    signaling profiles in the growing neural tube. In: Morphogen Gradients . Methods
    in Molecular Biology, vol. 1863, 47–63.'
  mla: Zagórski, Marcin P., and Anna Kicheva. “Measuring Dorsoventral Pattern and
    Morphogen Signaling Profiles in the Growing Neural Tube.” <i>Morphogen Gradients
    </i>, vol. 1863, Springer Nature, 2018, pp. 47–63, doi:<a href="https://doi.org/10.1007/978-1-4939-8772-6_4">10.1007/978-1-4939-8772-6_4</a>.
  short: M.P. Zagórski, A. Kicheva, in:, Morphogen Gradients , Springer Nature, 2018,
    pp. 47–63.
date_created: 2018-12-11T11:44:17Z
date_published: 2018-10-16T00:00:00Z
date_updated: 2025-04-14T07:27:29Z
day: '16'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1007/978-1-4939-8772-6_4
ec_funded: 1
file:
- access_level: open_access
  checksum: 2a97d0649fdcfcf1bdca7c8ad1dce71b
  content_type: application/pdf
  creator: dernst
  date_created: 2020-10-13T14:20:37Z
  date_updated: 2020-10-13T14:20:37Z
  file_id: '8656'
  file_name: 2018_MIMB_Zagorski.pdf
  file_size: 4906815
  relation: main_file
  success: 1
file_date_updated: 2020-10-13T14:20:37Z
has_accepted_license: '1'
intvolume: '      1863'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 47 - 63
project:
- _id: B6FC0238-B512-11E9-945C-1524E6697425
  call_identifier: H2020
  grant_number: '680037'
  name: Coordination of Patterning And Growth In the Spinal Cord
publication: 'Morphogen Gradients '
publication_identifier:
  isbn:
  - 978-1-4939-8771-9
  issn:
  - 1064-3745
publication_status: published
publisher: Springer Nature
publist_id: '8018'
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Measuring dorsoventral pattern and morphogen signaling profiles in the growing
  neural tube
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1863
year: '2018'
...