---
res:
  bibo_abstract:
  - 'Revealing the spectrum of combinatorial regulation of transcription at individual
    promoters is essential for understanding the complex structure of biological networks.
    However, the computations represented by the integration of various molecular
    signals at complex promoters are difficult to decipher in the absence of simple
    cis regulatory codes. Here we synthetically shuffle the regulatory architecture-operator
    sequences binding activators and repressors-of a canonical bacterial promoter.
    The resulting library of complex promoters allows for rapid exploration of promoter
    encoded logic regulation. Among all possible logic functions, NOR and ANDN promoter
    encoded logics predominate. A simple transcriptional cis regulatory code determines
    both logics, establishing a straightforward map between promoter structure and
    logic phenotype. The regulatory code is determined solely by the type of transcriptional
    regulation combinations: two repressors generate a NOR: NOT (a OR b) whereas a
    repressor and an activator generate an ANDN: a AND NOT b. Three-input versions
    of both logics, having an additional repressor as an input, are also present in
    the library. The resulting complex promoters cover a wide dynamic range of transcriptional
    strengths. Synthetic promoter shuffling represents a fast and efficient method
    for exploring the spectrum of complex regulatory functions that can be encoded
    by complex promoters. From an engineering point of view, synthetic promoter shuffling
    enables the experimental testing of the functional properties of complex promoters
    that cannot necessarily be inferred ab initio from the known properties of the
    individual genetic components. Synthetic promoter shuffling may provide a useful
    experimental tool for studying naturally occurring promoter shuffling.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Ali
      foaf_name: Kinkhabwala, Ali
      foaf_surname: Kinkhabwala
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Guet, Calin C
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  bibo_doi: 10.1371/journal.pone.0002030
  bibo_issue: '4'
  bibo_volume: 3
  dct_date: 2008^xs_gYear
  dct_language: eng
  dct_publisher: Public Library of Science@
  dct_title: Uncovering cis regulatory codes using synthetic promoter shuffling@
  fabio_hasPubmedId: '18446205'
...