---
res:
  bibo_abstract:
  - Characterizing the dynamics of specific RNA levels requires real-time RNA profiling
    in a single cell. We show that the combination of a synthetic modular genetic
    system with fluorescence correlation spectroscopy allows us to directly measure
    in real time the activity of any specific promoter in prokaryotes. Using a simple
    inducible gene expression system, we found that induced RNA levels within a single
    bacterium of Escherichia coli exhibited a pulsating profile in response to a steady
    input of inducer. The genetic deletion of an efflux pump system, a key determinant
    of antibiotic resistance, altered the pulsating transcriptional dynamics and caused
    overexpression of induced RNA. In contrast with population measurements, real-time
    RNA profiling permits identifying relationships between genotypes and transcriptional
    dynamics that are accessible only at the level of the single cell.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Thuc
      foaf_name: Le,Thuc T.
      foaf_surname: Le
  - foaf_Person:
      foaf_givenName: Sébastien
      foaf_name: Harlepp, Sébastien
      foaf_surname: Harlepp
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Calin Guet
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  - foaf_Person:
      foaf_givenName: Kimberly
      foaf_name: Dittmar,Kimberly
      foaf_surname: Dittmar
  - foaf_Person:
      foaf_givenName: Thierry
      foaf_name: Emonet,Thierry
      foaf_surname: Emonet
  - foaf_Person:
      foaf_givenName: Tao
      foaf_name: Pan,Tao
      foaf_surname: Pan
  - foaf_Person:
      foaf_givenName: Philippe
      foaf_name: Cluzel,Philippe
      foaf_surname: Cluzel
  bibo_doi: 10.1073/pnas.0503311102
  bibo_issue: '26'
  bibo_volume: 102
  dct_date: 2005^xs_gYear
  dct_publisher: National Academy of Sciences@
  dct_title: Real-time RNA profiling within a single bacterium@
...