[{"month":"01","title":"Real-time RNA profiling within a single bacterium","date_published":"2005-01-01T00:00:00Z","publisher":"National Academy of Sciences","date_updated":"2021-01-12T07:51:57Z","page":"9160 - 9164","abstract":[{"text":"Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell.","lang":"eng"}],"publication_status":"published","publist_id":"2476","_id":"3753","extern":1,"intvolume":"       102","author":[{"first_name":"Thuc","last_name":"Le","full_name":"Le,Thuc T."},{"last_name":"Harlepp","first_name":"Sébastien","full_name":"Harlepp, Sébastien"},{"first_name":"Calin C","last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","full_name":"Calin Guet"},{"full_name":"Dittmar,Kimberly","last_name":"Dittmar","first_name":"Kimberly"},{"last_name":"Emonet","first_name":"Thierry","full_name":"Emonet,Thierry"},{"last_name":"Pan","first_name":"Tao","full_name":"Pan,Tao"},{"last_name":"Cluzel","first_name":"Philippe","full_name":"Cluzel,Philippe"}],"acknowledgement":"4237","type":"journal_article","volume":102,"citation":{"ieee":"T. Le <i>et al.</i>, “Real-time RNA profiling within a single bacterium,” <i>PNAS</i>, vol. 102, no. 26. National Academy of Sciences, pp. 9160–9164, 2005.","short":"T. Le, S. Harlepp, C.C. Guet, K. Dittmar, T. Emonet, T. Pan, P. Cluzel, PNAS 102 (2005) 9160–9164.","ama":"Le T, Harlepp S, Guet CC, et al. Real-time RNA profiling within a single bacterium. <i>PNAS</i>. 2005;102(26):9160-9164. doi:<a href=\"https://doi.org/10.1073/pnas.0503311102\">10.1073/pnas.0503311102</a>","ista":"Le T, Harlepp S, Guet CC, Dittmar K, Emonet T, Pan T, Cluzel P. 2005. Real-time RNA profiling within a single bacterium. PNAS. 102(26), 9160–9164.","apa":"Le, T., Harlepp, S., Guet, C. C., Dittmar, K., Emonet, T., Pan, T., &#38; Cluzel, P. (2005). Real-time RNA profiling within a single bacterium. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.0503311102\">https://doi.org/10.1073/pnas.0503311102</a>","chicago":"Le, Thuc, Sébastien Harlepp, Calin C Guet, Kimberly Dittmar, Thierry Emonet, Tao Pan, and Philippe Cluzel. “Real-Time RNA Profiling within a Single Bacterium.” <i>PNAS</i>. National Academy of Sciences, 2005. <a href=\"https://doi.org/10.1073/pnas.0503311102\">https://doi.org/10.1073/pnas.0503311102</a>.","mla":"Le, Thuc, et al. “Real-Time RNA Profiling within a Single Bacterium.” <i>PNAS</i>, vol. 102, no. 26, National Academy of Sciences, 2005, pp. 9160–64, doi:<a href=\"https://doi.org/10.1073/pnas.0503311102\">10.1073/pnas.0503311102</a>."},"quality_controlled":0,"issue":"26","doi":"10.1073/pnas.0503311102","publication":"PNAS","status":"public","day":"01","year":"2005","date_created":"2018-12-11T12:04:59Z"}]